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Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice

Lymph nodes (LNs) are important sentinal organs, populated by circulating lymphocytes and antigen-bearing cells exiting the tissue beds. Although cellular and humoral immune responses are induced in LNs by antigenic challenge, it is not known if LNs are essential for acquired immunity. We examined i...

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Autores principales: Rennert, Paul D., Hochman, Paula S., Flavell, Richard A., Chaplin, David D., Jayaraman, Sundararajan, Browning, Jeffrey L., Fu, Yang-Xin
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193379/
https://www.ncbi.nlm.nih.gov/pubmed/11390430
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author Rennert, Paul D.
Hochman, Paula S.
Flavell, Richard A.
Chaplin, David D.
Jayaraman, Sundararajan
Browning, Jeffrey L.
Fu, Yang-Xin
author_facet Rennert, Paul D.
Hochman, Paula S.
Flavell, Richard A.
Chaplin, David D.
Jayaraman, Sundararajan
Browning, Jeffrey L.
Fu, Yang-Xin
author_sort Rennert, Paul D.
collection PubMed
description Lymph nodes (LNs) are important sentinal organs, populated by circulating lymphocytes and antigen-bearing cells exiting the tissue beds. Although cellular and humoral immune responses are induced in LNs by antigenic challenge, it is not known if LNs are essential for acquired immunity. We examined immune responses in mice that lack LNs due to genetic deletion of lymphotoxin ligands or in utero blockade of membrane lymphotoxin. We report that LNs are absolutely required for generating contact hypersensitivity, a T cell–dependent cellular immune response induced by epicutaneous hapten. We show that the homing of epidermal Langerhans cells in response to hapten application is specifically directed to LNs, providing a cellular basis for this unique LN function. In contrast, the spleen cannot mediate contact hypersensitivity because antigen-bearing epidermal Langerhans cells do not access splenic white pulp. Finally, we formally demonstrate that LNs provide a unique environment essential for generating this acquired immune response by reversing the LN defect in lymphotoxin-α(−/)− mice, thereby restoring the capacity for contact hypersensitivity.
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spelling pubmed-21933792008-04-14 Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice Rennert, Paul D. Hochman, Paula S. Flavell, Richard A. Chaplin, David D. Jayaraman, Sundararajan Browning, Jeffrey L. Fu, Yang-Xin J Exp Med Original Article Lymph nodes (LNs) are important sentinal organs, populated by circulating lymphocytes and antigen-bearing cells exiting the tissue beds. Although cellular and humoral immune responses are induced in LNs by antigenic challenge, it is not known if LNs are essential for acquired immunity. We examined immune responses in mice that lack LNs due to genetic deletion of lymphotoxin ligands or in utero blockade of membrane lymphotoxin. We report that LNs are absolutely required for generating contact hypersensitivity, a T cell–dependent cellular immune response induced by epicutaneous hapten. We show that the homing of epidermal Langerhans cells in response to hapten application is specifically directed to LNs, providing a cellular basis for this unique LN function. In contrast, the spleen cannot mediate contact hypersensitivity because antigen-bearing epidermal Langerhans cells do not access splenic white pulp. Finally, we formally demonstrate that LNs provide a unique environment essential for generating this acquired immune response by reversing the LN defect in lymphotoxin-α(−/)− mice, thereby restoring the capacity for contact hypersensitivity. The Rockefeller University Press 2001-06-04 /pmc/articles/PMC2193379/ /pubmed/11390430 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Rennert, Paul D.
Hochman, Paula S.
Flavell, Richard A.
Chaplin, David D.
Jayaraman, Sundararajan
Browning, Jeffrey L.
Fu, Yang-Xin
Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice
title Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice
title_full Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice
title_fullStr Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice
title_full_unstemmed Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice
title_short Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice
title_sort essential role of lymph nodes in contact hypersensitivity revealed in lymphotoxin-α–deficient mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193379/
https://www.ncbi.nlm.nih.gov/pubmed/11390430
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