Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis

Long-term cultured pre-B cells are able to differentiate into immunoglobulin (Ig)M-positive B cells (IgM(+) cells) when transplanted into severe combined immunodeficient (SCID) mice. Based on previous studies, here we report the development of a reconstitution assay in nonobese diabetic/SCID (NOD/SC...

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Detalles Bibliográficos
Autores principales: Ruiz-Vela, Antonio, Serrano, Fernando, González, Manuel A., Abad, José Luis, Bernad, Antonio, Maki, Masatoshi, Martínez-A, Carlos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193469/
https://www.ncbi.nlm.nih.gov/pubmed/11489944
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author Ruiz-Vela, Antonio
Serrano, Fernando
González, Manuel A.
Abad, José Luis
Bernad, Antonio
Maki, Masatoshi
Martínez-A, Carlos
author_facet Ruiz-Vela, Antonio
Serrano, Fernando
González, Manuel A.
Abad, José Luis
Bernad, Antonio
Maki, Masatoshi
Martínez-A, Carlos
author_sort Ruiz-Vela, Antonio
collection PubMed
description Long-term cultured pre-B cells are able to differentiate into immunoglobulin (Ig)M-positive B cells (IgM(+) cells) when transplanted into severe combined immunodeficient (SCID) mice. Based on previous studies, here we report the development of a reconstitution assay in nonobese diabetic/SCID (NOD/SCID) mice using pre-B cells, which allows us to study the role of calpains (calcium-activated endopeptidases) during B cell development as well as in B cell clonal deletion. Using this model, we show that calpastatin (the natural inhibitor of calpains) inhibits B cell receptor–induced apoptosis in IgM(+) cells derived from transplanted mice. We thus hypothesize an important function for calpain in sculpting the B cell repertoire.
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spelling pubmed-21934692008-04-14 Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis Ruiz-Vela, Antonio Serrano, Fernando González, Manuel A. Abad, José Luis Bernad, Antonio Maki, Masatoshi Martínez-A, Carlos J Exp Med Original Article Long-term cultured pre-B cells are able to differentiate into immunoglobulin (Ig)M-positive B cells (IgM(+) cells) when transplanted into severe combined immunodeficient (SCID) mice. Based on previous studies, here we report the development of a reconstitution assay in nonobese diabetic/SCID (NOD/SCID) mice using pre-B cells, which allows us to study the role of calpains (calcium-activated endopeptidases) during B cell development as well as in B cell clonal deletion. Using this model, we show that calpastatin (the natural inhibitor of calpains) inhibits B cell receptor–induced apoptosis in IgM(+) cells derived from transplanted mice. We thus hypothesize an important function for calpain in sculpting the B cell repertoire. The Rockefeller University Press 2001-08-06 /pmc/articles/PMC2193469/ /pubmed/11489944 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Ruiz-Vela, Antonio
Serrano, Fernando
González, Manuel A.
Abad, José Luis
Bernad, Antonio
Maki, Masatoshi
Martínez-A, Carlos
Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis
title Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis
title_full Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis
title_fullStr Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis
title_full_unstemmed Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis
title_short Transplanted Long-Term Cultured Pre-Bi Cells Expressing Calpastatin Are Resistant to B Cell Receptor–Induced Apoptosis
title_sort transplanted long-term cultured pre-bi cells expressing calpastatin are resistant to b cell receptor–induced apoptosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193469/
https://www.ncbi.nlm.nih.gov/pubmed/11489944
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