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Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells

It has been proposed that two different antigen-experienced T cell subsets may be distinguishable by their preferential ability to home to lymphoid organs (central memory cells) or nonlymphoid tissues (effector memory/effector cells). We have shown recently that murine antigen-primed CD8(+) T cells...

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Detalles Bibliográficos
Autores principales: Weninger, Wolfgang, Crowley, Maura A., Manjunath, N., von Andrian, Ulrich H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193483/
https://www.ncbi.nlm.nih.gov/pubmed/11581317
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author Weninger, Wolfgang
Crowley, Maura A.
Manjunath, N.
von Andrian, Ulrich H.
author_facet Weninger, Wolfgang
Crowley, Maura A.
Manjunath, N.
von Andrian, Ulrich H.
author_sort Weninger, Wolfgang
collection PubMed
description It has been proposed that two different antigen-experienced T cell subsets may be distinguishable by their preferential ability to home to lymphoid organs (central memory cells) or nonlymphoid tissues (effector memory/effector cells). We have shown recently that murine antigen-primed CD8(+) T cells cultured in interleukin (IL)-15 (CD8(IL-15)) resemble central memory cells in phenotype and function. In contrast, primed CD8(+) T cells cultured in IL-2 (CD8(IL-2)) become cytotoxic effector cells. Here, the migratory behavior of these two subsets was investigated. Naive, CD8(IL-15) cells and, to a lesser degree, CD8(IL-2) cells localized to T cell areas in the spleen, but only naive and CD8(IL-15) cells homed to lymph nodes (LNs) and Peyer's patches. Intravital microscopy of peripheral LNs revealed that CD8(IL-15) cells, but not CD8(IL-2) cells, rolled and arrested in high endothelial venules (HEVs). Migration of CD8(IL-15) cells to LNs depended on L-selectin and required chemokines that bind CC chemokine receptor (CCR)7. Both antigen-experienced populations, but not naive T cells, responded to inflammatory chemokines and accumulated at sites of inflammation. However, CD8(IL-2) cells were 12 times more efficient in migrating to inflamed peritoneum than CD8(IL-15) cells. Furthermore, CD8(IL-15) cells proliferated rapidly upon reencounter with antigen at sites of inflammation. Thus, central memory-like CD8(IL-15) cells home avidly to lymphoid organs and moderately to sites of inflammation, where they mediate rapid recall responses, whereas CD8(IL-2) effector T cells accumulate in inflamed tissues, but are excluded from most lymphoid organs.
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spelling pubmed-21934832008-04-14 Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells Weninger, Wolfgang Crowley, Maura A. Manjunath, N. von Andrian, Ulrich H. J Exp Med Original Article It has been proposed that two different antigen-experienced T cell subsets may be distinguishable by their preferential ability to home to lymphoid organs (central memory cells) or nonlymphoid tissues (effector memory/effector cells). We have shown recently that murine antigen-primed CD8(+) T cells cultured in interleukin (IL)-15 (CD8(IL-15)) resemble central memory cells in phenotype and function. In contrast, primed CD8(+) T cells cultured in IL-2 (CD8(IL-2)) become cytotoxic effector cells. Here, the migratory behavior of these two subsets was investigated. Naive, CD8(IL-15) cells and, to a lesser degree, CD8(IL-2) cells localized to T cell areas in the spleen, but only naive and CD8(IL-15) cells homed to lymph nodes (LNs) and Peyer's patches. Intravital microscopy of peripheral LNs revealed that CD8(IL-15) cells, but not CD8(IL-2) cells, rolled and arrested in high endothelial venules (HEVs). Migration of CD8(IL-15) cells to LNs depended on L-selectin and required chemokines that bind CC chemokine receptor (CCR)7. Both antigen-experienced populations, but not naive T cells, responded to inflammatory chemokines and accumulated at sites of inflammation. However, CD8(IL-2) cells were 12 times more efficient in migrating to inflamed peritoneum than CD8(IL-15) cells. Furthermore, CD8(IL-15) cells proliferated rapidly upon reencounter with antigen at sites of inflammation. Thus, central memory-like CD8(IL-15) cells home avidly to lymphoid organs and moderately to sites of inflammation, where they mediate rapid recall responses, whereas CD8(IL-2) effector T cells accumulate in inflamed tissues, but are excluded from most lymphoid organs. The Rockefeller University Press 2001-10-01 /pmc/articles/PMC2193483/ /pubmed/11581317 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Weninger, Wolfgang
Crowley, Maura A.
Manjunath, N.
von Andrian, Ulrich H.
Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells
title Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells
title_full Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells
title_fullStr Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells
title_full_unstemmed Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells
title_short Migratory Properties of Naive, Effector, and Memory Cd8(+) T Cells
title_sort migratory properties of naive, effector, and memory cd8(+) t cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193483/
https://www.ncbi.nlm.nih.gov/pubmed/11581317
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