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Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells

Synaptotagmins (Syts) I and II are believed to act as Ca(2+) sensors in the control of neurotransmission. Here we demonstrate that mast cells express Syt II in their lysosomal fraction. We further show that activation of mast cells by either aggregation of FcεRI or by Ca(2+) ionophores results in ex...

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Detalles Bibliográficos
Autores principales: Baram, Dana, Adachi, Roberto, Medalia, Ora, Tuvim, Michael, Dickey, Burton F., Mekori, Yoseph A., Sagi-Eisenberg, Ronit
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193646/
https://www.ncbi.nlm.nih.gov/pubmed/10330444
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author Baram, Dana
Adachi, Roberto
Medalia, Ora
Tuvim, Michael
Dickey, Burton F.
Mekori, Yoseph A.
Sagi-Eisenberg, Ronit
author_facet Baram, Dana
Adachi, Roberto
Medalia, Ora
Tuvim, Michael
Dickey, Burton F.
Mekori, Yoseph A.
Sagi-Eisenberg, Ronit
author_sort Baram, Dana
collection PubMed
description Synaptotagmins (Syts) I and II are believed to act as Ca(2+) sensors in the control of neurotransmission. Here we demonstrate that mast cells express Syt II in their lysosomal fraction. We further show that activation of mast cells by either aggregation of FcεRI or by Ca(2+) ionophores results in exocytosis of lysosomes, in addition to the well documented exocytosis of their secretory granules. Syt II directly regulates lysosomal exocytosis, whereby overexpression of Syt II inhibited Ca(2+)-triggered release of the lysosomal processed form of cathepsin D, whereas suppression of Syt II expression markedly potentiated this release. These findings provide evidence for a novel function of Syt II in negatively regulating Ca(2+)-triggered exocytosis of lysosomes, and suggest that Syt II–regulated secretion from lysosomes may play an important role in mast cell biology.
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spelling pubmed-21936462008-04-16 Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells Baram, Dana Adachi, Roberto Medalia, Ora Tuvim, Michael Dickey, Burton F. Mekori, Yoseph A. Sagi-Eisenberg, Ronit J Exp Med Articles Synaptotagmins (Syts) I and II are believed to act as Ca(2+) sensors in the control of neurotransmission. Here we demonstrate that mast cells express Syt II in their lysosomal fraction. We further show that activation of mast cells by either aggregation of FcεRI or by Ca(2+) ionophores results in exocytosis of lysosomes, in addition to the well documented exocytosis of their secretory granules. Syt II directly regulates lysosomal exocytosis, whereby overexpression of Syt II inhibited Ca(2+)-triggered release of the lysosomal processed form of cathepsin D, whereas suppression of Syt II expression markedly potentiated this release. These findings provide evidence for a novel function of Syt II in negatively regulating Ca(2+)-triggered exocytosis of lysosomes, and suggest that Syt II–regulated secretion from lysosomes may play an important role in mast cell biology. The Rockefeller University Press 1999-05-17 /pmc/articles/PMC2193646/ /pubmed/10330444 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Baram, Dana
Adachi, Roberto
Medalia, Ora
Tuvim, Michael
Dickey, Burton F.
Mekori, Yoseph A.
Sagi-Eisenberg, Ronit
Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells
title Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells
title_full Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells
title_fullStr Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells
title_full_unstemmed Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells
title_short Synaptotagmin II Negatively Regulates Ca(2+)-triggered Exocytosis of Lysosomes in Mast Cells
title_sort synaptotagmin ii negatively regulates ca(2+)-triggered exocytosis of lysosomes in mast cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193646/
https://www.ncbi.nlm.nih.gov/pubmed/10330444
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