Cargando…
Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System
The CDR3 regions of T cell receptor (TCR)-α and -β chains play central roles in the recognition of antigen (Ag)-MHC complex. TCR repertoire is created on the basis of Ag recognition specificity by CDR3s. To analyze the potential spectrum of TCR-α and -β to exhibit Ag specificity and generate TCR rep...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193687/ https://www.ncbi.nlm.nih.gov/pubmed/11956290 http://dx.doi.org/10.1084/jem.20010809 |
_version_ | 1782147528489172992 |
---|---|
author | Yokosuka, Tadashi Takase, Kan Suzuki, Misao Nakagawa, Yohko Taki, Shinsuke Takahashi, Hidemi Fujisawa, Takehiko Arase, Hisashi Saito, Takashi |
author_facet | Yokosuka, Tadashi Takase, Kan Suzuki, Misao Nakagawa, Yohko Taki, Shinsuke Takahashi, Hidemi Fujisawa, Takehiko Arase, Hisashi Saito, Takashi |
author_sort | Yokosuka, Tadashi |
collection | PubMed |
description | The CDR3 regions of T cell receptor (TCR)-α and -β chains play central roles in the recognition of antigen (Ag)-MHC complex. TCR repertoire is created on the basis of Ag recognition specificity by CDR3s. To analyze the potential spectrum of TCR-α and -β to exhibit Ag specificity and generate TCR repertoire, we established hundreds of TCR transfectants bearing a single TCR-α or -β chain derived from a cytotoxic T cell (CTL) clone, RT-1, specific for HIVgp160 peptide, and randomly picked up TCR-β or -α chains. Surprisingly, one-third of such TCR-β containing random CDR3β from naive T cells of normal mice could reconstitute the antigen-reactive TCR coupling with RT-1 TCR-α. A similar dominant function of TCR-α in forming Ag-specific TCR, though low-frequency, was obtained for lymphocytic choriomeningitis virus–specific TCR. Subsequently, we generated TCR-α and/or -β transgenic (Tg) mice specific for HIVgp160 peptide, and analyzed the TCR repertoire of Ag-specific CTLs. Similar to the results from TCR reconstitution, TCR-α Tg generated CTLs with heterogeneous TCR-β, whereas TCR-β Tg-induced CTLs bearing a single TCR-α. These findings of Ag recognition with minimum involvement of CDR3β expand our understanding regarding the flexibility of the spectrum of TCR and suggest a predominant role of TCR-α chain in determining the preimmune repertoire of Ag-specific TCR. |
format | Text |
id | pubmed-2193687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21936872008-04-14 Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System Yokosuka, Tadashi Takase, Kan Suzuki, Misao Nakagawa, Yohko Taki, Shinsuke Takahashi, Hidemi Fujisawa, Takehiko Arase, Hisashi Saito, Takashi J Exp Med Article The CDR3 regions of T cell receptor (TCR)-α and -β chains play central roles in the recognition of antigen (Ag)-MHC complex. TCR repertoire is created on the basis of Ag recognition specificity by CDR3s. To analyze the potential spectrum of TCR-α and -β to exhibit Ag specificity and generate TCR repertoire, we established hundreds of TCR transfectants bearing a single TCR-α or -β chain derived from a cytotoxic T cell (CTL) clone, RT-1, specific for HIVgp160 peptide, and randomly picked up TCR-β or -α chains. Surprisingly, one-third of such TCR-β containing random CDR3β from naive T cells of normal mice could reconstitute the antigen-reactive TCR coupling with RT-1 TCR-α. A similar dominant function of TCR-α in forming Ag-specific TCR, though low-frequency, was obtained for lymphocytic choriomeningitis virus–specific TCR. Subsequently, we generated TCR-α and/or -β transgenic (Tg) mice specific for HIVgp160 peptide, and analyzed the TCR repertoire of Ag-specific CTLs. Similar to the results from TCR reconstitution, TCR-α Tg generated CTLs with heterogeneous TCR-β, whereas TCR-β Tg-induced CTLs bearing a single TCR-α. These findings of Ag recognition with minimum involvement of CDR3β expand our understanding regarding the flexibility of the spectrum of TCR and suggest a predominant role of TCR-α chain in determining the preimmune repertoire of Ag-specific TCR. The Rockefeller University Press 2002-04-15 /pmc/articles/PMC2193687/ /pubmed/11956290 http://dx.doi.org/10.1084/jem.20010809 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Yokosuka, Tadashi Takase, Kan Suzuki, Misao Nakagawa, Yohko Taki, Shinsuke Takahashi, Hidemi Fujisawa, Takehiko Arase, Hisashi Saito, Takashi Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System |
title | Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System |
title_full | Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System |
title_fullStr | Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System |
title_full_unstemmed | Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System |
title_short | Predominant Role of T Cell Receptor (TCR)-α Chain in Forming Preimmune TCR Repertoire Revealed by Clonal TCR Reconstitution System |
title_sort | predominant role of t cell receptor (tcr)-α chain in forming preimmune tcr repertoire revealed by clonal tcr reconstitution system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193687/ https://www.ncbi.nlm.nih.gov/pubmed/11956290 http://dx.doi.org/10.1084/jem.20010809 |
work_keys_str_mv | AT yokosukatadashi predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT takasekan predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT suzukimisao predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT nakagawayohko predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT takishinsuke predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT takahashihidemi predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT fujisawatakehiko predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT arasehisashi predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem AT saitotakashi predominantroleoftcellreceptortcrachaininformingpreimmunetcrrepertoirerevealedbyclonaltcrreconstitutionsystem |