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Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses
Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and bu...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193767/ https://www.ncbi.nlm.nih.gov/pubmed/11877482 http://dx.doi.org/10.1084/jem.20011500 |
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author | Bavari, Sina Bosio, Catharine M. Wiegand, Elizabeth Ruthel, Gordon Will, Amy B. Geisbert, Thomas W. Hevey, Michael Schmaljohn, Connie Schmaljohn, Alan Aman, M. Javad |
author_facet | Bavari, Sina Bosio, Catharine M. Wiegand, Elizabeth Ruthel, Gordon Will, Amy B. Geisbert, Thomas W. Hevey, Michael Schmaljohn, Connie Schmaljohn, Alan Aman, M. Javad |
author_sort | Bavari, Sina |
collection | PubMed |
description | Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses. |
format | Text |
id | pubmed-2193767 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21937672008-04-14 Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses Bavari, Sina Bosio, Catharine M. Wiegand, Elizabeth Ruthel, Gordon Will, Amy B. Geisbert, Thomas W. Hevey, Michael Schmaljohn, Connie Schmaljohn, Alan Aman, M. Javad J Exp Med Original Article Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses. The Rockefeller University Press 2002-03-04 /pmc/articles/PMC2193767/ /pubmed/11877482 http://dx.doi.org/10.1084/jem.20011500 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Bavari, Sina Bosio, Catharine M. Wiegand, Elizabeth Ruthel, Gordon Will, Amy B. Geisbert, Thomas W. Hevey, Michael Schmaljohn, Connie Schmaljohn, Alan Aman, M. Javad Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses |
title | Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses |
title_full | Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses |
title_fullStr | Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses |
title_full_unstemmed | Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses |
title_short | Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses |
title_sort | lipid raft microdomains: a gateway for compartmentalized trafficking of ebola and marburg viruses |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193767/ https://www.ncbi.nlm.nih.gov/pubmed/11877482 http://dx.doi.org/10.1084/jem.20011500 |
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