Cargando…
A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion
Minor histocompatibility antigens (minor H antigens) are targets of graft-versus-host disease and graft-versus-leukemia responses after allogeneic human leukocyte antigen identical hematopoietic stem cell transplantation. Only a few human minor H antigens have been molecularly characterized and in a...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2003
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193779/ https://www.ncbi.nlm.nih.gov/pubmed/12743171 http://dx.doi.org/10.1084/jem.20030044 |
_version_ | 1782147550341496832 |
---|---|
author | Murata, Makoto Warren, Edus H. Riddell, Stanley R. |
author_facet | Murata, Makoto Warren, Edus H. Riddell, Stanley R. |
author_sort | Murata, Makoto |
collection | PubMed |
description | Minor histocompatibility antigens (minor H antigens) are targets of graft-versus-host disease and graft-versus-leukemia responses after allogeneic human leukocyte antigen identical hematopoietic stem cell transplantation. Only a few human minor H antigens have been molecularly characterized and in all cases, amino acid differences between homologous donor and recipient proteins due to nucleotide polymorphisms in the respective genes were responsible for immunogenicity. Here, we have used cDNA expression cloning to identify a novel human minor H antigen encoded by UGT2B17, an autosomal gene in the multigene UDP-glycosyltransferase 2 family that is selectively expressed in liver, intestine, and antigen-presenting cells. In contrast to previously defined human minor H antigens, UGT2B17 is immunogenic because of differential expression of the protein in donor and recipient cells as a consequence of a homozygous gene deletion in the donor. Deletion of individual members of large gene families is a common form of genetic variation in the population and our results provide the first evidence that differential protein expression as a consequence of gene deletion is a mechanism for generating minor H antigens in humans. |
format | Text |
id | pubmed-2193779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21937792008-04-11 A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion Murata, Makoto Warren, Edus H. Riddell, Stanley R. J Exp Med Article Minor histocompatibility antigens (minor H antigens) are targets of graft-versus-host disease and graft-versus-leukemia responses after allogeneic human leukocyte antigen identical hematopoietic stem cell transplantation. Only a few human minor H antigens have been molecularly characterized and in all cases, amino acid differences between homologous donor and recipient proteins due to nucleotide polymorphisms in the respective genes were responsible for immunogenicity. Here, we have used cDNA expression cloning to identify a novel human minor H antigen encoded by UGT2B17, an autosomal gene in the multigene UDP-glycosyltransferase 2 family that is selectively expressed in liver, intestine, and antigen-presenting cells. In contrast to previously defined human minor H antigens, UGT2B17 is immunogenic because of differential expression of the protein in donor and recipient cells as a consequence of a homozygous gene deletion in the donor. Deletion of individual members of large gene families is a common form of genetic variation in the population and our results provide the first evidence that differential protein expression as a consequence of gene deletion is a mechanism for generating minor H antigens in humans. The Rockefeller University Press 2003-05-19 /pmc/articles/PMC2193779/ /pubmed/12743171 http://dx.doi.org/10.1084/jem.20030044 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Murata, Makoto Warren, Edus H. Riddell, Stanley R. A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion |
title | A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion |
title_full | A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion |
title_fullStr | A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion |
title_full_unstemmed | A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion |
title_short | A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion |
title_sort | human minor histocompatibility antigen resulting from differential expression due to a gene deletion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193779/ https://www.ncbi.nlm.nih.gov/pubmed/12743171 http://dx.doi.org/10.1084/jem.20030044 |
work_keys_str_mv | AT muratamakoto ahumanminorhistocompatibilityantigenresultingfromdifferentialexpressionduetoagenedeletion AT warrenedush ahumanminorhistocompatibilityantigenresultingfromdifferentialexpressionduetoagenedeletion AT riddellstanleyr ahumanminorhistocompatibilityantigenresultingfromdifferentialexpressionduetoagenedeletion AT muratamakoto humanminorhistocompatibilityantigenresultingfromdifferentialexpressionduetoagenedeletion AT warrenedush humanminorhistocompatibilityantigenresultingfromdifferentialexpressionduetoagenedeletion AT riddellstanleyr humanminorhistocompatibilityantigenresultingfromdifferentialexpressionduetoagenedeletion |