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Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens

We report the identification of two novel minor histocompatibility antigens (mHAgs), encoded by two separate single nucleotide polymorphisms on a single gene, BCL2A1, and restricted by human histocompatibility leukocyte antigen (HLA)-A*2402 (the most common HLA-A allele in Japanese) and B*4403, resp...

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Autores principales: Akatsuka, Yoshiki, Nishida, Tetsuya, Kondo, Eisei, Miyazaki, Mikinori, Taji, Hirohumi, Iida, Hiroatsu, Tsujimura, Kunio, Yazaki, Makoto, Naoe, Tomoki, Morishima, Yasuo, Kodera, Yoshihisa, Kuzushima, Kiyotaka, Takahashi, Toshitada
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193899/
https://www.ncbi.nlm.nih.gov/pubmed/12771180
http://dx.doi.org/10.1084/jem.20021925
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author Akatsuka, Yoshiki
Nishida, Tetsuya
Kondo, Eisei
Miyazaki, Mikinori
Taji, Hirohumi
Iida, Hiroatsu
Tsujimura, Kunio
Yazaki, Makoto
Naoe, Tomoki
Morishima, Yasuo
Kodera, Yoshihisa
Kuzushima, Kiyotaka
Takahashi, Toshitada
author_facet Akatsuka, Yoshiki
Nishida, Tetsuya
Kondo, Eisei
Miyazaki, Mikinori
Taji, Hirohumi
Iida, Hiroatsu
Tsujimura, Kunio
Yazaki, Makoto
Naoe, Tomoki
Morishima, Yasuo
Kodera, Yoshihisa
Kuzushima, Kiyotaka
Takahashi, Toshitada
author_sort Akatsuka, Yoshiki
collection PubMed
description We report the identification of two novel minor histocompatibility antigens (mHAgs), encoded by two separate single nucleotide polymorphisms on a single gene, BCL2A1, and restricted by human histocompatibility leukocyte antigen (HLA)-A*2402 (the most common HLA-A allele in Japanese) and B*4403, respectively. Two cytotoxic T lymphocyte (CTL) clones specific for these mHAgs were first isolated from two distinct recipients after hematopoietic cell transplantation. Both clones lyse only normal and malignant cells within the hematopoietic lineage. To localize the gene encoding the mHAgs, two-point linkage analysis was performed on the CTL lytic patterns of restricting HLA-transfected B lymphoblastoid cell lines obtained from Centre d'Etude du Polymorphisme Humain. Both CTL clones showed a completely identical lytic pattern for 4 pedigrees and the gene was localized within a 3.6-cM interval of 15q24.3–25.1 region that encodes at least 46 genes. Of those, only BCL2A1 has been reported to be expressed in hematopoietic cells and possess three nonsynonymous nucleotide changes. Minigene transfection and epitope reconstitution assays with synthetic peptides identified both HLA-A*2402– and B*4403-restricted mHAg epitopes to be encoded by distinct polymorphisms within BCL2A1.
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spelling pubmed-21938992008-04-11 Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens Akatsuka, Yoshiki Nishida, Tetsuya Kondo, Eisei Miyazaki, Mikinori Taji, Hirohumi Iida, Hiroatsu Tsujimura, Kunio Yazaki, Makoto Naoe, Tomoki Morishima, Yasuo Kodera, Yoshihisa Kuzushima, Kiyotaka Takahashi, Toshitada J Exp Med Article We report the identification of two novel minor histocompatibility antigens (mHAgs), encoded by two separate single nucleotide polymorphisms on a single gene, BCL2A1, and restricted by human histocompatibility leukocyte antigen (HLA)-A*2402 (the most common HLA-A allele in Japanese) and B*4403, respectively. Two cytotoxic T lymphocyte (CTL) clones specific for these mHAgs were first isolated from two distinct recipients after hematopoietic cell transplantation. Both clones lyse only normal and malignant cells within the hematopoietic lineage. To localize the gene encoding the mHAgs, two-point linkage analysis was performed on the CTL lytic patterns of restricting HLA-transfected B lymphoblastoid cell lines obtained from Centre d'Etude du Polymorphisme Humain. Both CTL clones showed a completely identical lytic pattern for 4 pedigrees and the gene was localized within a 3.6-cM interval of 15q24.3–25.1 region that encodes at least 46 genes. Of those, only BCL2A1 has been reported to be expressed in hematopoietic cells and possess three nonsynonymous nucleotide changes. Minigene transfection and epitope reconstitution assays with synthetic peptides identified both HLA-A*2402– and B*4403-restricted mHAg epitopes to be encoded by distinct polymorphisms within BCL2A1. The Rockefeller University Press 2003-06-02 /pmc/articles/PMC2193899/ /pubmed/12771180 http://dx.doi.org/10.1084/jem.20021925 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Akatsuka, Yoshiki
Nishida, Tetsuya
Kondo, Eisei
Miyazaki, Mikinori
Taji, Hirohumi
Iida, Hiroatsu
Tsujimura, Kunio
Yazaki, Makoto
Naoe, Tomoki
Morishima, Yasuo
Kodera, Yoshihisa
Kuzushima, Kiyotaka
Takahashi, Toshitada
Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens
title Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens
title_full Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens
title_fullStr Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens
title_full_unstemmed Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens
title_short Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens
title_sort identification of a polymorphic gene, bcl2a1, encoding two novel hematopoietic lineage-specific minor histocompatibility antigens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193899/
https://www.ncbi.nlm.nih.gov/pubmed/12771180
http://dx.doi.org/10.1084/jem.20021925
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