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Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma

We have used cDNA arrays to investigate gene expression patterns in peripheral blood mononuclear cells from patients with leukemic forms of cutaneous T cell lymphoma, primarily Sezary syndrome (SS). When expression data for patients with high blood tumor burden (Sezary cells >60% of the lymphocyt...

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Autores principales: Kari, Laszlo, Loboda, Andrey, Nebozhyn, Michael, Rook, Alain H., Vonderheid, Eric C., Nichols, Calen, Virok, Dezso, Chang, Celia, Horng, Wen-Hwai, Johnston, James, Wysocka, Maria, Showe, Michael K., Showe, Louise C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193901/
https://www.ncbi.nlm.nih.gov/pubmed/12782714
http://dx.doi.org/10.1084/jem.20021726
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author Kari, Laszlo
Loboda, Andrey
Nebozhyn, Michael
Rook, Alain H.
Vonderheid, Eric C.
Nichols, Calen
Virok, Dezso
Chang, Celia
Horng, Wen-Hwai
Johnston, James
Wysocka, Maria
Showe, Michael K.
Showe, Louise C.
author_facet Kari, Laszlo
Loboda, Andrey
Nebozhyn, Michael
Rook, Alain H.
Vonderheid, Eric C.
Nichols, Calen
Virok, Dezso
Chang, Celia
Horng, Wen-Hwai
Johnston, James
Wysocka, Maria
Showe, Michael K.
Showe, Louise C.
author_sort Kari, Laszlo
collection PubMed
description We have used cDNA arrays to investigate gene expression patterns in peripheral blood mononuclear cells from patients with leukemic forms of cutaneous T cell lymphoma, primarily Sezary syndrome (SS). When expression data for patients with high blood tumor burden (Sezary cells >60% of the lymphocytes) and healthy controls are compared by Student's t test, at P < 0.01, we find 385 genes to be differentially expressed. Highly overexpressed genes include Th2 cells–specific transcription factors Gata-3 and Jun B, as well as integrin β1, proteoglycan 2, the RhoB oncogene, and dual specificity phosphatase 1. Highly underexpressed genes include CD26, Stat-4, and the IL-1 receptors. Message for plastin-T, not normally expressed in lymphoid tissue, is detected only in patient samples and may provide a new marker for diagnosis. Using penalized discriminant analysis, we have identified a panel of eight genes that can distinguish SS in patients with as few as 5% circulating tumor cells. This suggests that, even in early disease, Sezary cells produce chemokines and cytokines that induce an expression profile in the peripheral blood distinctive to SS. Finally, we show that using 10 genes, we can identify a class of patients who will succumb within six months of sampling regardless of their tumor burden.
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spelling pubmed-21939012008-04-11 Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma Kari, Laszlo Loboda, Andrey Nebozhyn, Michael Rook, Alain H. Vonderheid, Eric C. Nichols, Calen Virok, Dezso Chang, Celia Horng, Wen-Hwai Johnston, James Wysocka, Maria Showe, Michael K. Showe, Louise C. J Exp Med Article We have used cDNA arrays to investigate gene expression patterns in peripheral blood mononuclear cells from patients with leukemic forms of cutaneous T cell lymphoma, primarily Sezary syndrome (SS). When expression data for patients with high blood tumor burden (Sezary cells >60% of the lymphocytes) and healthy controls are compared by Student's t test, at P < 0.01, we find 385 genes to be differentially expressed. Highly overexpressed genes include Th2 cells–specific transcription factors Gata-3 and Jun B, as well as integrin β1, proteoglycan 2, the RhoB oncogene, and dual specificity phosphatase 1. Highly underexpressed genes include CD26, Stat-4, and the IL-1 receptors. Message for plastin-T, not normally expressed in lymphoid tissue, is detected only in patient samples and may provide a new marker for diagnosis. Using penalized discriminant analysis, we have identified a panel of eight genes that can distinguish SS in patients with as few as 5% circulating tumor cells. This suggests that, even in early disease, Sezary cells produce chemokines and cytokines that induce an expression profile in the peripheral blood distinctive to SS. Finally, we show that using 10 genes, we can identify a class of patients who will succumb within six months of sampling regardless of their tumor burden. The Rockefeller University Press 2003-06-02 /pmc/articles/PMC2193901/ /pubmed/12782714 http://dx.doi.org/10.1084/jem.20021726 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kari, Laszlo
Loboda, Andrey
Nebozhyn, Michael
Rook, Alain H.
Vonderheid, Eric C.
Nichols, Calen
Virok, Dezso
Chang, Celia
Horng, Wen-Hwai
Johnston, James
Wysocka, Maria
Showe, Michael K.
Showe, Louise C.
Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma
title Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma
title_full Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma
title_fullStr Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma
title_full_unstemmed Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma
title_short Classification and Prediction of Survival in Patients with the Leukemic Phase of Cutaneous T Cell Lymphoma
title_sort classification and prediction of survival in patients with the leukemic phase of cutaneous t cell lymphoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193901/
https://www.ncbi.nlm.nih.gov/pubmed/12782714
http://dx.doi.org/10.1084/jem.20021726
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