Cargando…

Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements

Immunoglobulin light chain (IgL) rearrangements occur more frequently at Igκ than at Igλ. Previous results suggested that the unrearranged Igκ locus negatively regulates Igλ transcription and/or rearrangement. Here, we demonstrate that expression of a VJλ1-joint inserted into its physiological posit...

Descripción completa

Detalles Bibliográficos
Autores principales: Oberdoerffer, Philipp, Novobrantseva, Tatiana I., Rajewsky, Klaus
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193966/
https://www.ncbi.nlm.nih.gov/pubmed/12719477
http://dx.doi.org/10.1084/jem.20030402
_version_ 1782147594455089152
author Oberdoerffer, Philipp
Novobrantseva, Tatiana I.
Rajewsky, Klaus
author_facet Oberdoerffer, Philipp
Novobrantseva, Tatiana I.
Rajewsky, Klaus
author_sort Oberdoerffer, Philipp
collection PubMed
description Immunoglobulin light chain (IgL) rearrangements occur more frequently at Igκ than at Igλ. Previous results suggested that the unrearranged Igκ locus negatively regulates Igλ transcription and/or rearrangement. Here, we demonstrate that expression of a VJλ1-joint inserted into its physiological position in the Igλ locus is independent of Igκ rearrangements. Expression of the inserted VJλ1 gene segment is developmentally controlled like that of a VJκ-joint inserted into the Igκ locus and furthermore coincides developmentally with the occurrence of Igκ rearrangements in wild-type mice. We conclude that developmentally controlled transcription of a gene rearrangement in the Igλ locus occurs in the presence of an unrearranged Igκ locus and is therefore not negatively regulated by the latter. Our data also indicate light chain editing in ∼30% of λ1 expressing B cell progenitors.
format Text
id pubmed-2193966
institution National Center for Biotechnology Information
language English
publishDate 2003
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21939662008-04-11 Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements Oberdoerffer, Philipp Novobrantseva, Tatiana I. Rajewsky, Klaus J Exp Med Article Immunoglobulin light chain (IgL) rearrangements occur more frequently at Igκ than at Igλ. Previous results suggested that the unrearranged Igκ locus negatively regulates Igλ transcription and/or rearrangement. Here, we demonstrate that expression of a VJλ1-joint inserted into its physiological position in the Igλ locus is independent of Igκ rearrangements. Expression of the inserted VJλ1 gene segment is developmentally controlled like that of a VJκ-joint inserted into the Igκ locus and furthermore coincides developmentally with the occurrence of Igκ rearrangements in wild-type mice. We conclude that developmentally controlled transcription of a gene rearrangement in the Igλ locus occurs in the presence of an unrearranged Igκ locus and is therefore not negatively regulated by the latter. Our data also indicate light chain editing in ∼30% of λ1 expressing B cell progenitors. The Rockefeller University Press 2003-05-05 /pmc/articles/PMC2193966/ /pubmed/12719477 http://dx.doi.org/10.1084/jem.20030402 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Oberdoerffer, Philipp
Novobrantseva, Tatiana I.
Rajewsky, Klaus
Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements
title Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements
title_full Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements
title_fullStr Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements
title_full_unstemmed Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements
title_short Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements
title_sort expression of a targeted λ1 light chain gene is developmentally regulated and independent of igκ rearrangements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193966/
https://www.ncbi.nlm.nih.gov/pubmed/12719477
http://dx.doi.org/10.1084/jem.20030402
work_keys_str_mv AT oberdoerfferphilipp expressionofatargetedl1lightchaingeneisdevelopmentallyregulatedandindependentofigkrearrangements
AT novobrantsevatatianai expressionofatargetedl1lightchaingeneisdevelopmentallyregulatedandindependentofigkrearrangements
AT rajewskyklaus expressionofatargetedl1lightchaingeneisdevelopmentallyregulatedandindependentofigkrearrangements