Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches

B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4(−/−) B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but...

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Autores principales: Okada, Takaharu, Ngo, Vu N., Ekland, Eric H., Förster, Reinhold, Lipp, Martin, Littman, Dan R., Cyster, Jason G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194009/
https://www.ncbi.nlm.nih.gov/pubmed/12093871
http://dx.doi.org/10.1084/jem.20020201
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author Okada, Takaharu
Ngo, Vu N.
Ekland, Eric H.
Förster, Reinhold
Lipp, Martin
Littman, Dan R.
Cyster, Jason G.
author_facet Okada, Takaharu
Ngo, Vu N.
Ekland, Eric H.
Förster, Reinhold
Lipp, Martin
Littman, Dan R.
Cyster, Jason G.
author_sort Okada, Takaharu
collection PubMed
description B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4(−/−) B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches.
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spelling pubmed-21940092008-04-11 Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches Okada, Takaharu Ngo, Vu N. Ekland, Eric H. Förster, Reinhold Lipp, Martin Littman, Dan R. Cyster, Jason G. J Exp Med Article B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4(−/−) B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches. The Rockefeller University Press 2002-07-01 /pmc/articles/PMC2194009/ /pubmed/12093871 http://dx.doi.org/10.1084/jem.20020201 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Okada, Takaharu
Ngo, Vu N.
Ekland, Eric H.
Förster, Reinhold
Lipp, Martin
Littman, Dan R.
Cyster, Jason G.
Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches
title Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches
title_full Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches
title_fullStr Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches
title_full_unstemmed Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches
title_short Chemokine Requirements for B Cell Entry to Lymph Nodes and Peyer's Patches
title_sort chemokine requirements for b cell entry to lymph nodes and peyer's patches
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194009/
https://www.ncbi.nlm.nih.gov/pubmed/12093871
http://dx.doi.org/10.1084/jem.20020201
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