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Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice

Control of infection with virulent Mycobacterium tuberculosis (Mtb) in mice is dependent on the generation of T helper (Th)1-mediated immunity that serves, via secretion of interferon (IFN)-γ and other cytokines, to upregulate the antimycobacterial function of macrophages of which the synthesis of i...

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Autores principales: Jung, Yu-Jin, LaCourse, Ronald, Ryan, Lynn, North, Robert J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194026/
https://www.ncbi.nlm.nih.gov/pubmed/12370260
http://dx.doi.org/10.1084/jem.20021186
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author Jung, Yu-Jin
LaCourse, Ronald
Ryan, Lynn
North, Robert J.
author_facet Jung, Yu-Jin
LaCourse, Ronald
Ryan, Lynn
North, Robert J.
author_sort Jung, Yu-Jin
collection PubMed
description Control of infection with virulent Mycobacterium tuberculosis (Mtb) in mice is dependent on the generation of T helper (Th)1-mediated immunity that serves, via secretion of interferon (IFN)-γ and other cytokines, to upregulate the antimycobacterial function of macrophages of which the synthesis of inducible nitric oxide synthase (NOS)2 is an essential event. As a means to understanding the basis of Mtb virulence, the ability of gene-deleted mice incapable of making NOS2 (NOS2(−/−)), gp91(Phox) subunit of the respiratory burst NADPH-oxidase complex (Phox(−/−)), or either enzyme (NOS2/Phox(−/−)), to control airborne infection with the avirulent R1Rv and H37Ra strains of Mtb was compared with their ability control infection with the virulent H37Rv strain. NOS2(−/−), Phox(−/−), and NOS2/Phox(−/−) mice showed no deficiency in ability to control infection with either strain of avirulent Mtb. By contrast, NOS2(−/−) mice, but not Phox(−/−) mice, were incapable of controlling H37Rv infection and died early from neutrophil-dominated lung pathology. Control of infection with avirulent, as well as virulent Mtb, depended on the synthesis of IFN-γ, and was associated with a substantial increase in the synthesis in the lungs of mRNA for IFN-γ and NOS2, and with production of NOS2 by macrophages at sites of infection. The results indicate that virulent, but not avirulent, Mtb can overcome the growth inhibitory action of a Th1–dependent, NOS2-independent mechanism of defense.
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spelling pubmed-21940262008-04-11 Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice Jung, Yu-Jin LaCourse, Ronald Ryan, Lynn North, Robert J. J Exp Med Article Control of infection with virulent Mycobacterium tuberculosis (Mtb) in mice is dependent on the generation of T helper (Th)1-mediated immunity that serves, via secretion of interferon (IFN)-γ and other cytokines, to upregulate the antimycobacterial function of macrophages of which the synthesis of inducible nitric oxide synthase (NOS)2 is an essential event. As a means to understanding the basis of Mtb virulence, the ability of gene-deleted mice incapable of making NOS2 (NOS2(−/−)), gp91(Phox) subunit of the respiratory burst NADPH-oxidase complex (Phox(−/−)), or either enzyme (NOS2/Phox(−/−)), to control airborne infection with the avirulent R1Rv and H37Ra strains of Mtb was compared with their ability control infection with the virulent H37Rv strain. NOS2(−/−), Phox(−/−), and NOS2/Phox(−/−) mice showed no deficiency in ability to control infection with either strain of avirulent Mtb. By contrast, NOS2(−/−) mice, but not Phox(−/−) mice, were incapable of controlling H37Rv infection and died early from neutrophil-dominated lung pathology. Control of infection with avirulent, as well as virulent Mtb, depended on the synthesis of IFN-γ, and was associated with a substantial increase in the synthesis in the lungs of mRNA for IFN-γ and NOS2, and with production of NOS2 by macrophages at sites of infection. The results indicate that virulent, but not avirulent, Mtb can overcome the growth inhibitory action of a Th1–dependent, NOS2-independent mechanism of defense. The Rockefeller University Press 2002-10-07 /pmc/articles/PMC2194026/ /pubmed/12370260 http://dx.doi.org/10.1084/jem.20021186 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Jung, Yu-Jin
LaCourse, Ronald
Ryan, Lynn
North, Robert J.
Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice
title Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice
title_full Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice
title_fullStr Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice
title_full_unstemmed Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice
title_short Virulent but not Avirulent Mycobacterium tuberculosis Can Evade the Growth Inhibitory Action of a T Helper 1–dependent, Nitric Oxide Synthase 2–independent Defense in Mice
title_sort virulent but not avirulent mycobacterium tuberculosis can evade the growth inhibitory action of a t helper 1–dependent, nitric oxide synthase 2–independent defense in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194026/
https://www.ncbi.nlm.nih.gov/pubmed/12370260
http://dx.doi.org/10.1084/jem.20021186
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