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Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo

Murine splenic dendritic cells (DCs) can be divided into two subsets based on CD8α expression, but the specific role of each subset in stimulation of T cells is largely unknown. An important function of DCs is the ability to take up exogenous antigens and cross-present them in the context of major h...

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Autores principales: den Haan, Joke M.M., Bevan, Michael J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194052/
https://www.ncbi.nlm.nih.gov/pubmed/12235214
http://dx.doi.org/10.1084/jem.20020295
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author den Haan, Joke M.M.
Bevan, Michael J.
author_facet den Haan, Joke M.M.
Bevan, Michael J.
author_sort den Haan, Joke M.M.
collection PubMed
description Murine splenic dendritic cells (DCs) can be divided into two subsets based on CD8α expression, but the specific role of each subset in stimulation of T cells is largely unknown. An important function of DCs is the ability to take up exogenous antigens and cross-present them in the context of major histocompatibility complex (MHC) class I molecules to CD8(+) T cells. We previously demonstrated that, when cell-associated ovalbumin (OVA) is injected into mice, only the CD8(+) DC subset cross-presents OVA in the context of MHC class I. In contrast to this selectivity with cell-associated antigen, we show here that both DC subsets isolated from mice injected with OVA/anti-OVA immune complexes (OVA-IC) cross-present OVA to CD8(+) T cells. The use of immunoglobulin G Fc receptor (FcγR) common γ-chain–deficient mice revealed that the cross-presentation by CD8(−) DCs depended on the expression of γ-chain–containing activating FcγRs, whereas cross-presentation by CD8(+) DCs was not reduced in γ-chain–deficient mice. These results suggest that although CD8(+) DCs constitutively cross-present exogenous antigens in the context of MHC class I molecules, CD8(−) DCs only do so after activation, such as via ligation of FcγRs. Cross-presentation of immune complexes may play an important role in autoimmune diseases and the therapeutic effect of antitumor antibodies.
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spelling pubmed-21940522008-04-11 Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo den Haan, Joke M.M. Bevan, Michael J. J Exp Med Article Murine splenic dendritic cells (DCs) can be divided into two subsets based on CD8α expression, but the specific role of each subset in stimulation of T cells is largely unknown. An important function of DCs is the ability to take up exogenous antigens and cross-present them in the context of major histocompatibility complex (MHC) class I molecules to CD8(+) T cells. We previously demonstrated that, when cell-associated ovalbumin (OVA) is injected into mice, only the CD8(+) DC subset cross-presents OVA in the context of MHC class I. In contrast to this selectivity with cell-associated antigen, we show here that both DC subsets isolated from mice injected with OVA/anti-OVA immune complexes (OVA-IC) cross-present OVA to CD8(+) T cells. The use of immunoglobulin G Fc receptor (FcγR) common γ-chain–deficient mice revealed that the cross-presentation by CD8(−) DCs depended on the expression of γ-chain–containing activating FcγRs, whereas cross-presentation by CD8(+) DCs was not reduced in γ-chain–deficient mice. These results suggest that although CD8(+) DCs constitutively cross-present exogenous antigens in the context of MHC class I molecules, CD8(−) DCs only do so after activation, such as via ligation of FcγRs. Cross-presentation of immune complexes may play an important role in autoimmune diseases and the therapeutic effect of antitumor antibodies. The Rockefeller University Press 2002-09-16 /pmc/articles/PMC2194052/ /pubmed/12235214 http://dx.doi.org/10.1084/jem.20020295 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
den Haan, Joke M.M.
Bevan, Michael J.
Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo
title Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo
title_full Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo
title_fullStr Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo
title_full_unstemmed Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo
title_short Constitutive versus Activation-dependent Cross-Presentation of Immune Complexes by CD8(+) and CD8(−) Dendritic Cells In Vivo
title_sort constitutive versus activation-dependent cross-presentation of immune complexes by cd8(+) and cd8(−) dendritic cells in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194052/
https://www.ncbi.nlm.nih.gov/pubmed/12235214
http://dx.doi.org/10.1084/jem.20020295
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