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Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone

The marginal zone of the spleen is a precisely ordered region that contains specialized subsets of B lymphocytes and macrophages. Disruption of the negative signaling inositol phosphatase, SH2-containing inositol-5-phosphatase 1 (SHIP), results in the loss of marginal zone B cells (MZBs) with reorga...

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Autores principales: Karlsson, Mikael C.I., Guinamard, Rodolphe, Bolland, Silvia, Sankala, Marko, Steinman, Ralph M., Ravetch, Jeffrey V.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194070/
https://www.ncbi.nlm.nih.gov/pubmed/12874264
http://dx.doi.org/10.1084/jem.20030684
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author Karlsson, Mikael C.I.
Guinamard, Rodolphe
Bolland, Silvia
Sankala, Marko
Steinman, Ralph M.
Ravetch, Jeffrey V.
author_facet Karlsson, Mikael C.I.
Guinamard, Rodolphe
Bolland, Silvia
Sankala, Marko
Steinman, Ralph M.
Ravetch, Jeffrey V.
author_sort Karlsson, Mikael C.I.
collection PubMed
description The marginal zone of the spleen is a precisely ordered region that contains specialized subsets of B lymphocytes and macrophages. Disruption of the negative signaling inositol phosphatase, SH2-containing inositol-5-phosphatase 1 (SHIP), results in the loss of marginal zone B cells (MZBs) with reorganization of marginal zone macrophages (MZMOs) to the red pulp of the spleen. This primary macrophage defect, as revealed by selectively depleting SHIP in myeloid cells shows that MZMOs are specifically required for the retention of MZBs. The MZMO phenotype was reverted in SHIP/Bruton's tyrosine kinase (Btk) double knockout mice, thus identifying the Btk activating pathway as an essential component being regulated by SHIP. Furthermore, we identified a direct interaction between the MARCO scavenger receptor on MZMOs and MZBs. Activation or disruption of this interaction results in MZB migration to the follicle. The migration of the MZMOs was further studied after the response to Staphylococcus aureus, which induced MZMOs to move into the red pulp while MZBs migrated into the follicular zone. The marginal zone is therefore a dynamic structure in which retention and trafficking of B cells requires specific macrophage–B cell interactions.
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spelling pubmed-21940702008-04-11 Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone Karlsson, Mikael C.I. Guinamard, Rodolphe Bolland, Silvia Sankala, Marko Steinman, Ralph M. Ravetch, Jeffrey V. J Exp Med Article The marginal zone of the spleen is a precisely ordered region that contains specialized subsets of B lymphocytes and macrophages. Disruption of the negative signaling inositol phosphatase, SH2-containing inositol-5-phosphatase 1 (SHIP), results in the loss of marginal zone B cells (MZBs) with reorganization of marginal zone macrophages (MZMOs) to the red pulp of the spleen. This primary macrophage defect, as revealed by selectively depleting SHIP in myeloid cells shows that MZMOs are specifically required for the retention of MZBs. The MZMO phenotype was reverted in SHIP/Bruton's tyrosine kinase (Btk) double knockout mice, thus identifying the Btk activating pathway as an essential component being regulated by SHIP. Furthermore, we identified a direct interaction between the MARCO scavenger receptor on MZMOs and MZBs. Activation or disruption of this interaction results in MZB migration to the follicle. The migration of the MZMOs was further studied after the response to Staphylococcus aureus, which induced MZMOs to move into the red pulp while MZBs migrated into the follicular zone. The marginal zone is therefore a dynamic structure in which retention and trafficking of B cells requires specific macrophage–B cell interactions. The Rockefeller University Press 2003-07-21 /pmc/articles/PMC2194070/ /pubmed/12874264 http://dx.doi.org/10.1084/jem.20030684 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Karlsson, Mikael C.I.
Guinamard, Rodolphe
Bolland, Silvia
Sankala, Marko
Steinman, Ralph M.
Ravetch, Jeffrey V.
Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone
title Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone
title_full Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone
title_fullStr Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone
title_full_unstemmed Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone
title_short Macrophages Control the Retention and Trafficking of B Lymphocytes in the Splenic Marginal Zone
title_sort macrophages control the retention and trafficking of b lymphocytes in the splenic marginal zone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194070/
https://www.ncbi.nlm.nih.gov/pubmed/12874264
http://dx.doi.org/10.1084/jem.20030684
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