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The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression
Pax5 activity is enhanced in activated B cells and is essential for class switch recombination (CSR). We show that inhibitor of differentiation (Id)2 suppresses CSR by repressing the gene expression of activation-induced cytidine deaminase (AID), which has been shown to be indispensable for CSR. Fur...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194241/ https://www.ncbi.nlm.nih.gov/pubmed/14581609 http://dx.doi.org/10.1084/jem.20030802 |
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author | Gonda, Hiroyuki Sugai, Manabu Nambu, Yukiko Katakai, Tomoya Agata, Yasutoshi Mori, Kazuhiro J. Yokota, Yoshifumi Shimizu, Akira |
author_facet | Gonda, Hiroyuki Sugai, Manabu Nambu, Yukiko Katakai, Tomoya Agata, Yasutoshi Mori, Kazuhiro J. Yokota, Yoshifumi Shimizu, Akira |
author_sort | Gonda, Hiroyuki |
collection | PubMed |
description | Pax5 activity is enhanced in activated B cells and is essential for class switch recombination (CSR). We show that inhibitor of differentiation (Id)2 suppresses CSR by repressing the gene expression of activation-induced cytidine deaminase (AID), which has been shown to be indispensable for CSR. Furthermore, a putative regulatory region of AID contains E2A- and Pax5-binding sites, and the latter site is indispensable for AID gene expression. Moreover, the DNA-binding activity of Pax5 is decreased in Id2-overexpressing B cells and enhanced in Id2(−/−) B cells. The kinetics of Pax5, but not E2A, occupancy to AID locus is the same as AID expression in primary B cells. Finally, enforced expression of Pax5 induces AID transcription in pro–B cell lines. Our results provide evidence that the balance between Pax5 and Id2 activities has a key role in AID gene expression. |
format | Text |
id | pubmed-2194241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21942412008-04-11 The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression Gonda, Hiroyuki Sugai, Manabu Nambu, Yukiko Katakai, Tomoya Agata, Yasutoshi Mori, Kazuhiro J. Yokota, Yoshifumi Shimizu, Akira J Exp Med Article Pax5 activity is enhanced in activated B cells and is essential for class switch recombination (CSR). We show that inhibitor of differentiation (Id)2 suppresses CSR by repressing the gene expression of activation-induced cytidine deaminase (AID), which has been shown to be indispensable for CSR. Furthermore, a putative regulatory region of AID contains E2A- and Pax5-binding sites, and the latter site is indispensable for AID gene expression. Moreover, the DNA-binding activity of Pax5 is decreased in Id2-overexpressing B cells and enhanced in Id2(−/−) B cells. The kinetics of Pax5, but not E2A, occupancy to AID locus is the same as AID expression in primary B cells. Finally, enforced expression of Pax5 induces AID transcription in pro–B cell lines. Our results provide evidence that the balance between Pax5 and Id2 activities has a key role in AID gene expression. The Rockefeller University Press 2003-11-03 /pmc/articles/PMC2194241/ /pubmed/14581609 http://dx.doi.org/10.1084/jem.20030802 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Gonda, Hiroyuki Sugai, Manabu Nambu, Yukiko Katakai, Tomoya Agata, Yasutoshi Mori, Kazuhiro J. Yokota, Yoshifumi Shimizu, Akira The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression |
title | The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression |
title_full | The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression |
title_fullStr | The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression |
title_full_unstemmed | The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression |
title_short | The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression |
title_sort | balance between pax5 and id2 activities is the key to aid gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194241/ https://www.ncbi.nlm.nih.gov/pubmed/14581609 http://dx.doi.org/10.1084/jem.20030802 |
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