Cargando…

Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility

Mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection. Numerous intracellular pathogens, including Listeria monocytogenes, Shigella flexneri, and Rickettsia ric...

Descripción completa

Detalles Bibliográficos
Autores principales: Stamm, Luisa M., Morisaki, J. Hiroshi, Gao, Lian-Yong, Jeng, Robert L., McDonald, Kent L., Roth, Robyn, Takeshita, Sunao, Heuser, John, Welch, Matthew D., Brown, Eric J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194249/
https://www.ncbi.nlm.nih.gov/pubmed/14597736
http://dx.doi.org/10.1084/jem.20031072
_version_ 1782147660789055488
author Stamm, Luisa M.
Morisaki, J. Hiroshi
Gao, Lian-Yong
Jeng, Robert L.
McDonald, Kent L.
Roth, Robyn
Takeshita, Sunao
Heuser, John
Welch, Matthew D.
Brown, Eric J.
author_facet Stamm, Luisa M.
Morisaki, J. Hiroshi
Gao, Lian-Yong
Jeng, Robert L.
McDonald, Kent L.
Roth, Robyn
Takeshita, Sunao
Heuser, John
Welch, Matthew D.
Brown, Eric J.
author_sort Stamm, Luisa M.
collection PubMed
description Mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection. Numerous intracellular pathogens, including Listeria monocytogenes, Shigella flexneri, and Rickettsia rickettsii, exploit the host cytoskeleton by using actin-based motility for cell to cell spread during infection. Here we show that Mycobacterium marinum, a natural pathogen of fish and frogs and an occasional pathogen of humans, is capable of actively inducing actin polymerization within macrophages. M. marinum that polymerized actin were free in the cytoplasm and propelled by actin-based motility into adjacent cells. Immunofluorescence demonstrated the presence of host cytoskeletal proteins, including the Arp2/3 complex and vasodilator-stimulated phosphoprotein, throughout the actin tails. In contrast, Wiskott-Aldrich syndrome protein localized exclusively at the actin-polymerizing pole of M. marinum. These findings show that M. marinum can escape into the cytoplasm of infected macrophages, where it can recruit host cell cytoskeletal factors to induce actin polymerization leading to direct cell to cell spread.
format Text
id pubmed-2194249
institution National Center for Biotechnology Information
language English
publishDate 2003
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21942492008-04-11 Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility Stamm, Luisa M. Morisaki, J. Hiroshi Gao, Lian-Yong Jeng, Robert L. McDonald, Kent L. Roth, Robyn Takeshita, Sunao Heuser, John Welch, Matthew D. Brown, Eric J. J Exp Med Article Mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection. Numerous intracellular pathogens, including Listeria monocytogenes, Shigella flexneri, and Rickettsia rickettsii, exploit the host cytoskeleton by using actin-based motility for cell to cell spread during infection. Here we show that Mycobacterium marinum, a natural pathogen of fish and frogs and an occasional pathogen of humans, is capable of actively inducing actin polymerization within macrophages. M. marinum that polymerized actin were free in the cytoplasm and propelled by actin-based motility into adjacent cells. Immunofluorescence demonstrated the presence of host cytoskeletal proteins, including the Arp2/3 complex and vasodilator-stimulated phosphoprotein, throughout the actin tails. In contrast, Wiskott-Aldrich syndrome protein localized exclusively at the actin-polymerizing pole of M. marinum. These findings show that M. marinum can escape into the cytoplasm of infected macrophages, where it can recruit host cell cytoskeletal factors to induce actin polymerization leading to direct cell to cell spread. The Rockefeller University Press 2003-11-03 /pmc/articles/PMC2194249/ /pubmed/14597736 http://dx.doi.org/10.1084/jem.20031072 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Stamm, Luisa M.
Morisaki, J. Hiroshi
Gao, Lian-Yong
Jeng, Robert L.
McDonald, Kent L.
Roth, Robyn
Takeshita, Sunao
Heuser, John
Welch, Matthew D.
Brown, Eric J.
Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility
title Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility
title_full Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility
title_fullStr Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility
title_full_unstemmed Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility
title_short Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility
title_sort mycobacterium marinum escapes from phagosomes and is propelled by actin-based motility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194249/
https://www.ncbi.nlm.nih.gov/pubmed/14597736
http://dx.doi.org/10.1084/jem.20031072
work_keys_str_mv AT stammluisam mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT morisakijhiroshi mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT gaolianyong mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT jengrobertl mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT mcdonaldkentl mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT rothrobyn mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT takeshitasunao mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT heuserjohn mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT welchmatthewd mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility
AT brownericj mycobacteriummarinumescapesfromphagosomesandispropelledbyactinbasedmotility