Cargando…

Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid h...

Descripción completa

Detalles Bibliográficos
Autores principales: Thiry, Julien, Widén, Frederik, Grégoire, Fabien, Linden, Annick, Belák, Sándor, Thiry, Etienne
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194762/
https://www.ncbi.nlm.nih.gov/pubmed/17903260
http://dx.doi.org/10.1186/1746-6148-3-26
_version_ 1782147689429860352
author Thiry, Julien
Widén, Frederik
Grégoire, Fabien
Linden, Annick
Belák, Sándor
Thiry, Etienne
author_facet Thiry, Julien
Widén, Frederik
Grégoire, Fabien
Linden, Annick
Belák, Sándor
Thiry, Etienne
author_sort Thiry, Julien
collection PubMed
description BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid herpesviruses 1 (CvHV-1) and 2 (CvHV-2) and elk herpesvirus 1 (ElkHV-1). Considering the serological relationship between these ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV-1 related virus infection in wild and domestic ruminant species. In this way, a recent investigation has indicated, in Belgium, a high increase in the serological prevalence of BoHV-1 related virus infection in free-ranging red deer population. In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population. RESULTS: The current study reports the first isolation in a free-ranging red deer of a BoHV-1 closely related virus. The isolate was antigenically, genomically and genetically characterised by comparison with several ruminant alphaherpesvirus. Immunofluorescence assays revealed the isolate was antigenically distinct from bovine and caprine alphaherpesviruses. Similarly, BamHI and BstEII restriction analyses demonstrated the genomic difference between the isolate and the other ruminant alphaherpesviruses. Next, the sequencing of selected parts of UL27 and US8 genes showed a high degree of homologies between each BoHV-1 related ruminant alphaherpesvirus and the isolate. Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1. CONCLUSION: The first isolation of a virus closely related to BoHV-1 in a free-ranging red deer is reported. Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe. Anlier strain show consistent differences with the virus isolated from Scottish farmed red deer. All together, these results improve our understanding of ruminant alphaherpesviruses.
format Text
id pubmed-2194762
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-21947622008-01-13 Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer Thiry, Julien Widén, Frederik Grégoire, Fabien Linden, Annick Belák, Sándor Thiry, Etienne BMC Vet Res Research Article BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid herpesviruses 1 (CvHV-1) and 2 (CvHV-2) and elk herpesvirus 1 (ElkHV-1). Considering the serological relationship between these ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV-1 related virus infection in wild and domestic ruminant species. In this way, a recent investigation has indicated, in Belgium, a high increase in the serological prevalence of BoHV-1 related virus infection in free-ranging red deer population. In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population. RESULTS: The current study reports the first isolation in a free-ranging red deer of a BoHV-1 closely related virus. The isolate was antigenically, genomically and genetically characterised by comparison with several ruminant alphaherpesvirus. Immunofluorescence assays revealed the isolate was antigenically distinct from bovine and caprine alphaherpesviruses. Similarly, BamHI and BstEII restriction analyses demonstrated the genomic difference between the isolate and the other ruminant alphaherpesviruses. Next, the sequencing of selected parts of UL27 and US8 genes showed a high degree of homologies between each BoHV-1 related ruminant alphaherpesvirus and the isolate. Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1. CONCLUSION: The first isolation of a virus closely related to BoHV-1 in a free-ranging red deer is reported. Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe. Anlier strain show consistent differences with the virus isolated from Scottish farmed red deer. All together, these results improve our understanding of ruminant alphaherpesviruses. BioMed Central 2007-09-28 /pmc/articles/PMC2194762/ /pubmed/17903260 http://dx.doi.org/10.1186/1746-6148-3-26 Text en Copyright © 2007 Thiry et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Thiry, Julien
Widén, Frederik
Grégoire, Fabien
Linden, Annick
Belák, Sándor
Thiry, Etienne
Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
title Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
title_full Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
title_fullStr Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
title_full_unstemmed Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
title_short Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
title_sort isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194762/
https://www.ncbi.nlm.nih.gov/pubmed/17903260
http://dx.doi.org/10.1186/1746-6148-3-26
work_keys_str_mv AT thiryjulien isolationandcharacterisationofaruminantalphaherpesviruscloselyrelatedtobovineherpesvirus1inafreerangingreddeer
AT widenfrederik isolationandcharacterisationofaruminantalphaherpesviruscloselyrelatedtobovineherpesvirus1inafreerangingreddeer
AT gregoirefabien isolationandcharacterisationofaruminantalphaherpesviruscloselyrelatedtobovineherpesvirus1inafreerangingreddeer
AT lindenannick isolationandcharacterisationofaruminantalphaherpesviruscloselyrelatedtobovineherpesvirus1inafreerangingreddeer
AT belaksandor isolationandcharacterisationofaruminantalphaherpesviruscloselyrelatedtobovineherpesvirus1inafreerangingreddeer
AT thiryetienne isolationandcharacterisationofaruminantalphaherpesviruscloselyrelatedtobovineherpesvirus1inafreerangingreddeer