Cargando…

DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES

The kinetics of movement of tracer Na into human and dog red cells have been studied. The time courses of these processes and of K transfer were compared with the theoretical time course for saturation of a flat sheet having a resistive surface. The theoretical and the experimental curves when plott...

Descripción completa

Detalles Bibliográficos
Autores principales: Harris, E. J., Prankerd, T. A. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1957
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194814/
https://www.ncbi.nlm.nih.gov/pubmed/13463277
_version_ 1782147699170082816
author Harris, E. J.
Prankerd, T. A. J.
author_facet Harris, E. J.
Prankerd, T. A. J.
author_sort Harris, E. J.
collection PubMed
description The kinetics of movement of tracer Na into human and dog red cells have been studied. The time courses of these processes and of K transfer were compared with the theoretical time course for saturation of a flat sheet having a resistive surface. The theoretical and the experimental curves when plotted against t (½) have a considerable portion which is linear; on the basis of this resemblance the results are interpreted in terms of a permeability constant and an internal diffusion constant. It is supposed that selective adsorption acts to bring about concentration of K in the human cell and that the bulk of the Na of that cell is present in a thin outer region, while the K is in the interior. The action of strophanthin is to remove the usual limit to the Na capacity of the cell and it is proposed that the Na region increases in thickness at the expense of the K region. Omission of K from the medium has a similar result. Na uptake into poisoned cells measured either with tracer or as a net gain has a linear dependence upon t (½) after a delay. The permeability of the dog cell to Na is reduced when K is added to the medium; this may be due to the formation of an outer K-rich region which imposes a resistance to Na movement.
format Text
id pubmed-2194814
institution National Center for Biotechnology Information
language English
publishDate 1957
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21948142008-04-23 DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES Harris, E. J. Prankerd, T. A. J. J Gen Physiol Article The kinetics of movement of tracer Na into human and dog red cells have been studied. The time courses of these processes and of K transfer were compared with the theoretical time course for saturation of a flat sheet having a resistive surface. The theoretical and the experimental curves when plotted against t (½) have a considerable portion which is linear; on the basis of this resemblance the results are interpreted in terms of a permeability constant and an internal diffusion constant. It is supposed that selective adsorption acts to bring about concentration of K in the human cell and that the bulk of the Na of that cell is present in a thin outer region, while the K is in the interior. The action of strophanthin is to remove the usual limit to the Na capacity of the cell and it is proposed that the Na region increases in thickness at the expense of the K region. Omission of K from the medium has a similar result. Na uptake into poisoned cells measured either with tracer or as a net gain has a linear dependence upon t (½) after a delay. The permeability of the dog cell to Na is reduced when K is added to the medium; this may be due to the formation of an outer K-rich region which imposes a resistance to Na movement. The Rockefeller University Press 1957-09-20 /pmc/articles/PMC2194814/ /pubmed/13463277 Text en Copyright © Copyright, 1957, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Harris, E. J.
Prankerd, T. A. J.
DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES
title DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES
title_full DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES
title_fullStr DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES
title_full_unstemmed DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES
title_short DIFFUSION AND PERMEATION OF CATIONS IN HUMAN AND DOG ERYTHROCYTES
title_sort diffusion and permeation of cations in human and dog erythrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194814/
https://www.ncbi.nlm.nih.gov/pubmed/13463277
work_keys_str_mv AT harrisej diffusionandpermeationofcationsinhumananddogerythrocytes
AT prankerdtaj diffusionandpermeationofcationsinhumananddogerythrocytes