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TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS
Synaptic transmission was studied in giant synapses of the stellate ganglion of the squid. When bathed in air-saturated sea water, the synapses deteriorate in 10 to 20 min.; if the sea water is saturated with 100 per cent oxygen, they function steadily for up to 12 hours. Optimal results probably re...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1958
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194846/ https://www.ncbi.nlm.nih.gov/pubmed/13491817 |
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author | Bryant, S. H. |
author_facet | Bryant, S. H. |
author_sort | Bryant, S. H. |
collection | PubMed |
description | Synaptic transmission was studied in giant synapses of the stellate ganglion of the squid. When bathed in air-saturated sea water, the synapses deteriorate in 10 to 20 min.; if the sea water is saturated with 100 per cent oxygen, they function steadily for up to 12 hours. Optimal results probably require a medium with lower magnesium and higher calcium than the sea water used. Of eighteen compounds known to affect other synapses (Table I), none had stimulatory effects when applied to the preparation, but ten produced synaptic depression in concentrations of 10(–3) gm. per ml. or higher. The only exception was procaine, which blocked at 6 x 10(–5) gm. per ml. Intracellular recording with microelectrodes near the synapse showed that the block was associated with a slower rise of the excitatory post-synaptic potential, without a change in the depolarization required to initiate the spike. Procaine was exceptional in also increasing the depolarization at which the spike occurred. |
format | Text |
id | pubmed-2194846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1958 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21948462008-04-23 TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS Bryant, S. H. J Gen Physiol Article Synaptic transmission was studied in giant synapses of the stellate ganglion of the squid. When bathed in air-saturated sea water, the synapses deteriorate in 10 to 20 min.; if the sea water is saturated with 100 per cent oxygen, they function steadily for up to 12 hours. Optimal results probably require a medium with lower magnesium and higher calcium than the sea water used. Of eighteen compounds known to affect other synapses (Table I), none had stimulatory effects when applied to the preparation, but ten produced synaptic depression in concentrations of 10(–3) gm. per ml. or higher. The only exception was procaine, which blocked at 6 x 10(–5) gm. per ml. Intracellular recording with microelectrodes near the synapse showed that the block was associated with a slower rise of the excitatory post-synaptic potential, without a change in the depolarization required to initiate the spike. Procaine was exceptional in also increasing the depolarization at which the spike occurred. The Rockefeller University Press 1958-01-20 /pmc/articles/PMC2194846/ /pubmed/13491817 Text en Copyright © Copyright, 1958, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Bryant, S. H. TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS |
title | TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS |
title_full | TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS |
title_fullStr | TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS |
title_full_unstemmed | TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS |
title_short | TRANSMISSION IN SQUID GIANT SYNAPSES : THE IMPORTANCE OF OXYGEN SUPPLY AND THE EFFECTS OF DRUGS |
title_sort | transmission in squid giant synapses : the importance of oxygen supply and the effects of drugs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194846/ https://www.ncbi.nlm.nih.gov/pubmed/13491817 |
work_keys_str_mv | AT bryantsh transmissioninsquidgiantsynapsestheimportanceofoxygensupplyandtheeffectsofdrugs |