AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT

An enzymatic ion exchange model for active sodium transport is described. Kinetic equations relating net flux to time, and to concentration difference across the actively transporting membrane are derived. The second of these equations is tested, using the isolated frog skin in the "short-circu...

Descripción completa

Detalles Bibliográficos
Autor principal: Koblick, Daniel C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194927/
https://www.ncbi.nlm.nih.gov/pubmed/13620892
_version_ 1782147725961199616
author Koblick, Daniel C.
author_facet Koblick, Daniel C.
author_sort Koblick, Daniel C.
collection PubMed
description An enzymatic ion exchange model for active sodium transport is described. Kinetic equations relating net flux to time, and to concentration difference across the actively transporting membrane are derived. The second of these equations is tested, using the isolated frog skin in the "short-circuit" apparatus of Ussing. Reasonable linearity, as predicted by this equation, is observed. The passive permeability coefficient for Na(+), is calculated as 5.3 x 10(-4) ± 5.3 x 10(-4) cm./hr. If cholinesterase is assumed to be the enzyme responsible for transport, the activity required to account for the observations reported here is 17.7 x 10(-4) mmoles/cm.(2)/hr.
format Text
id pubmed-2194927
institution National Center for Biotechnology Information
language English
publishDate 1959
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21949272008-04-23 AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT Koblick, Daniel C. J Gen Physiol Article An enzymatic ion exchange model for active sodium transport is described. Kinetic equations relating net flux to time, and to concentration difference across the actively transporting membrane are derived. The second of these equations is tested, using the isolated frog skin in the "short-circuit" apparatus of Ussing. Reasonable linearity, as predicted by this equation, is observed. The passive permeability coefficient for Na(+), is calculated as 5.3 x 10(-4) ± 5.3 x 10(-4) cm./hr. If cholinesterase is assumed to be the enzyme responsible for transport, the activity required to account for the observations reported here is 17.7 x 10(-4) mmoles/cm.(2)/hr. The Rockefeller University Press 1959-01-20 /pmc/articles/PMC2194927/ /pubmed/13620892 Text en Copyright © Copyright, 1959, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Koblick, Daniel C.
AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT
title AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT
title_full AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT
title_fullStr AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT
title_full_unstemmed AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT
title_short AN ENZYMATIC ION EXCHANGE MODEL FOR ACTIVE SODIUM TRANSPORT
title_sort enzymatic ion exchange model for active sodium transport
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194927/
https://www.ncbi.nlm.nih.gov/pubmed/13620892
work_keys_str_mv AT koblickdanielc anenzymaticionexchangemodelforactivesodiumtransport
AT koblickdanielc enzymaticionexchangemodelforactivesodiumtransport

Ejemplares similares