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Growth and Division of Single Cells of Higher Plants in Vitro

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones wh...

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Detalles Bibliográficos
Autor principal: Bergmann, Ludwig
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195034/
https://www.ncbi.nlm.nih.gov/pubmed/13799400
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author Bergmann, Ludwig
author_facet Bergmann, Ludwig
author_sort Bergmann, Ludwig
collection PubMed
description The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.
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spelling pubmed-21950342008-04-23 Growth and Division of Single Cells of Higher Plants in Vitro Bergmann, Ludwig J Gen Physiol Article The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope. The Rockefeller University Press 1960-03-01 /pmc/articles/PMC2195034/ /pubmed/13799400 Text en Copyright © Copyright, 1960, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bergmann, Ludwig
Growth and Division of Single Cells of Higher Plants in Vitro
title Growth and Division of Single Cells of Higher Plants in Vitro
title_full Growth and Division of Single Cells of Higher Plants in Vitro
title_fullStr Growth and Division of Single Cells of Higher Plants in Vitro
title_full_unstemmed Growth and Division of Single Cells of Higher Plants in Vitro
title_short Growth and Division of Single Cells of Higher Plants in Vitro
title_sort growth and division of single cells of higher plants in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195034/
https://www.ncbi.nlm.nih.gov/pubmed/13799400
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