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An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells
Lysis of human red cells in vitro by an enzyme obtained from rabbit red cell hemolysates and the inhibition of this lytic activity by human stroma have been shown to require Mg(++) and ATP, and ATP utilization has been demonstrated in both reactions. We find that sodium or potassium ions are also re...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1960
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195044/ https://www.ncbi.nlm.nih.gov/pubmed/13802745 |
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author | Borek, Blanche Ann Zaffuto, Stephen F. Bovarnick, Max |
author_facet | Borek, Blanche Ann Zaffuto, Stephen F. Bovarnick, Max |
author_sort | Borek, Blanche Ann |
collection | PubMed |
description | Lysis of human red cells in vitro by an enzyme obtained from rabbit red cell hemolysates and the inhibition of this lytic activity by human stroma have been shown to require Mg(++) and ATP, and ATP utilization has been demonstrated in both reactions. We find that sodium or potassium ions are also required for the lytic phenomenon and that they enhance the inhibition. The rate of hemolysis is not affected by the internal concentrations of these ions but depends only on the external concentration. The rate of influx and efflux of Na(22)Cl and K(42)Cl in surviving red cells is greatly enhanced both during and after treatment with rabbit hemolytic factor whereas the entry of C(24)-sucrose, a small foreign molecule, is mediated only in the presence of hemolytic factor. Glycolysis neither protects against lysis nor enhances the activity of this system, and cardiac glycosides which are known inhibitors of active transport of ions also have no effect. It appears that lysis in this system is not brought about by increased active transport of ions into the cell but that the rabbit factor degrades or combines with some membrane component, altering permeability and resulting in increased diffusion, first of sodium and potassium ions and other small molecules, and finally of large molecules (hemoglobin) out of the cell. |
format | Text |
id | pubmed-2195044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1960 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21950442008-04-23 An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells Borek, Blanche Ann Zaffuto, Stephen F. Bovarnick, Max J Gen Physiol Article Lysis of human red cells in vitro by an enzyme obtained from rabbit red cell hemolysates and the inhibition of this lytic activity by human stroma have been shown to require Mg(++) and ATP, and ATP utilization has been demonstrated in both reactions. We find that sodium or potassium ions are also required for the lytic phenomenon and that they enhance the inhibition. The rate of hemolysis is not affected by the internal concentrations of these ions but depends only on the external concentration. The rate of influx and efflux of Na(22)Cl and K(42)Cl in surviving red cells is greatly enhanced both during and after treatment with rabbit hemolytic factor whereas the entry of C(24)-sucrose, a small foreign molecule, is mediated only in the presence of hemolytic factor. Glycolysis neither protects against lysis nor enhances the activity of this system, and cardiac glycosides which are known inhibitors of active transport of ions also have no effect. It appears that lysis in this system is not brought about by increased active transport of ions into the cell but that the rabbit factor degrades or combines with some membrane component, altering permeability and resulting in increased diffusion, first of sodium and potassium ions and other small molecules, and finally of large molecules (hemoglobin) out of the cell. The Rockefeller University Press 1960-05-01 /pmc/articles/PMC2195044/ /pubmed/13802745 Text en Copyright © Copyright, 1960, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Borek, Blanche Ann Zaffuto, Stephen F. Bovarnick, Max An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells |
title | An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells |
title_full | An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells |
title_fullStr | An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells |
title_full_unstemmed | An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells |
title_short | An Adenosinetriphosphate-Activated Hemolytic System : III. Effect of rabbit hemolytic factor on the membrane permeability of human red cells |
title_sort | adenosinetriphosphate-activated hemolytic system : iii. effect of rabbit hemolytic factor on the membrane permeability of human red cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195044/ https://www.ncbi.nlm.nih.gov/pubmed/13802745 |
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