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The Surface of the Washed Human Erythrocyte as a Polyanion

The electrokinetic behaviour of normal erythrocytes is compared with that of trypsin-, N-bromosuccinimide-, and tosyl-treated erythrocytes. Reduction in the net negative charge with reduction in ionic strength of the suspending medium and also on treatment with N-bromosuccinimide and trypsin is disc...

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Autores principales: Seaman, G. V. F., Heard, D. H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195093/
https://www.ncbi.nlm.nih.gov/pubmed/13749485
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author Seaman, G. V. F.
Heard, D. H.
author_facet Seaman, G. V. F.
Heard, D. H.
author_sort Seaman, G. V. F.
collection PubMed
description The electrokinetic behaviour of normal erythrocytes is compared with that of trypsin-, N-bromosuccinimide-, and tosyl-treated erythrocytes. Reduction in the net negative charge with reduction in ionic strength of the suspending medium and also on treatment with N-bromosuccinimide and trypsin is discussed using a porous non-rigid polyanion as a model for the periphery of the cell membrane. It is deduced from the equivalent binding of chloride and thiocyanate ions and the absence of any effect on treatment of red cells with tosyl chloride, that normal, N-bromosuccinimide- and trypsin-treated cells are polyanionic in character. Reduction in erythrocyte charge on treatment with N-bromosuccinimide or trypsin is probably not due to the removal of phosphate groups from the interface, nor to physical adsorption of N-bromosuccinimide or trypsin. The charge reduction is probably produced by bond fission with possibly a net disappearance of carboxyl groups from the electrophoretic plane of shear either by loss from, or reorientation of, the membrane. The loss or reorientation of material associated with these carboxyl groups does not lead to any basic change in the character of the surface of the cell, nor to any obvious structural instability. The biconcave discoid form is maintained and there is no significant hemolysis of the erythrocytes even after contact with a solution of trypsin for 24 hours.
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spelling pubmed-21950932008-04-23 The Surface of the Washed Human Erythrocyte as a Polyanion Seaman, G. V. F. Heard, D. H. J Gen Physiol Article The electrokinetic behaviour of normal erythrocytes is compared with that of trypsin-, N-bromosuccinimide-, and tosyl-treated erythrocytes. Reduction in the net negative charge with reduction in ionic strength of the suspending medium and also on treatment with N-bromosuccinimide and trypsin is discussed using a porous non-rigid polyanion as a model for the periphery of the cell membrane. It is deduced from the equivalent binding of chloride and thiocyanate ions and the absence of any effect on treatment of red cells with tosyl chloride, that normal, N-bromosuccinimide- and trypsin-treated cells are polyanionic in character. Reduction in erythrocyte charge on treatment with N-bromosuccinimide or trypsin is probably not due to the removal of phosphate groups from the interface, nor to physical adsorption of N-bromosuccinimide or trypsin. The charge reduction is probably produced by bond fission with possibly a net disappearance of carboxyl groups from the electrophoretic plane of shear either by loss from, or reorientation of, the membrane. The loss or reorientation of material associated with these carboxyl groups does not lead to any basic change in the character of the surface of the cell, nor to any obvious structural instability. The biconcave discoid form is maintained and there is no significant hemolysis of the erythrocytes even after contact with a solution of trypsin for 24 hours. The Rockefeller University Press 1960-11-01 /pmc/articles/PMC2195093/ /pubmed/13749485 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Seaman, G. V. F.
Heard, D. H.
The Surface of the Washed Human Erythrocyte as a Polyanion
title The Surface of the Washed Human Erythrocyte as a Polyanion
title_full The Surface of the Washed Human Erythrocyte as a Polyanion
title_fullStr The Surface of the Washed Human Erythrocyte as a Polyanion
title_full_unstemmed The Surface of the Washed Human Erythrocyte as a Polyanion
title_short The Surface of the Washed Human Erythrocyte as a Polyanion
title_sort surface of the washed human erythrocyte as a polyanion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195093/
https://www.ncbi.nlm.nih.gov/pubmed/13749485
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