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Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells

The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotoni...

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Detalles Bibliográficos
Autores principales: Whittembury, Guillermo, Sugino, Nobuhiro, Solomon, A. K.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195119/
https://www.ncbi.nlm.nih.gov/pubmed/13784943
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author Whittembury, Guillermo
Sugino, Nobuhiro
Solomon, A. K.
author_facet Whittembury, Guillermo
Sugino, Nobuhiro
Solomon, A. K.
author_sort Whittembury, Guillermo
collection PubMed
description The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotonic solutions. The results of both methods were found to agree well. Measurements were also made of electrical potential differences across the peritubular face of the kidney cells using bathing solutions in which the electrolyte composition and concentrations could be varied. The data obtained are consistent with a model cell in which the potential difference arises as a result of differences in Na permeability relative to K on the two faces of the cell. The intracellular Na concentration is considered to be regulated by a Na-K coupled pump located at the peritubular face of the cell.
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spelling pubmed-21951192008-04-23 Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells Whittembury, Guillermo Sugino, Nobuhiro Solomon, A. K. J Gen Physiol Article The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotonic solutions. The results of both methods were found to agree well. Measurements were also made of electrical potential differences across the peritubular face of the kidney cells using bathing solutions in which the electrolyte composition and concentrations could be varied. The data obtained are consistent with a model cell in which the potential difference arises as a result of differences in Na permeability relative to K on the two faces of the cell. The intracellular Na concentration is considered to be regulated by a Na-K coupled pump located at the peritubular face of the cell. The Rockefeller University Press 1961-03-01 /pmc/articles/PMC2195119/ /pubmed/13784943 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Whittembury, Guillermo
Sugino, Nobuhiro
Solomon, A. K.
Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
title Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
title_full Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
title_fullStr Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
title_full_unstemmed Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
title_short Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
title_sort ionic permeability and electrical potential differences in necturus kidney cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195119/
https://www.ncbi.nlm.nih.gov/pubmed/13784943
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