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Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotoni...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1961
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195119/ https://www.ncbi.nlm.nih.gov/pubmed/13784943 |
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author | Whittembury, Guillermo Sugino, Nobuhiro Solomon, A. K. |
author_facet | Whittembury, Guillermo Sugino, Nobuhiro Solomon, A. K. |
author_sort | Whittembury, Guillermo |
collection | PubMed |
description | The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotonic solutions. The results of both methods were found to agree well. Measurements were also made of electrical potential differences across the peritubular face of the kidney cells using bathing solutions in which the electrolyte composition and concentrations could be varied. The data obtained are consistent with a model cell in which the potential difference arises as a result of differences in Na permeability relative to K on the two faces of the cell. The intracellular Na concentration is considered to be regulated by a Na-K coupled pump located at the peritubular face of the cell. |
format | Text |
id | pubmed-2195119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1961 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21951192008-04-23 Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells Whittembury, Guillermo Sugino, Nobuhiro Solomon, A. K. J Gen Physiol Article The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotonic solutions. The results of both methods were found to agree well. Measurements were also made of electrical potential differences across the peritubular face of the kidney cells using bathing solutions in which the electrolyte composition and concentrations could be varied. The data obtained are consistent with a model cell in which the potential difference arises as a result of differences in Na permeability relative to K on the two faces of the cell. The intracellular Na concentration is considered to be regulated by a Na-K coupled pump located at the peritubular face of the cell. The Rockefeller University Press 1961-03-01 /pmc/articles/PMC2195119/ /pubmed/13784943 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Whittembury, Guillermo Sugino, Nobuhiro Solomon, A. K. Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells |
title | Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells |
title_full | Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells |
title_fullStr | Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells |
title_full_unstemmed | Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells |
title_short | Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells |
title_sort | ionic permeability and electrical potential differences in necturus kidney cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195119/ https://www.ncbi.nlm.nih.gov/pubmed/13784943 |
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