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Factors Controlling the Production of Lysogenic Cultures of B. megatherium
(1) The proportion of infected B. megatherium cells which develop lysogenic colonies depends on the number and kind of infecting virus particles and on the culture medium in which the cells are growing. (2) Cells infected with 100 or more T virus particles (from megatherium 899) in yeast extract pep...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1961
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195128/ https://www.ncbi.nlm.nih.gov/pubmed/13729631 |
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author | Northrop, John H. |
author_facet | Northrop, John H. |
author_sort | Northrop, John H. |
collection | PubMed |
description | (1) The proportion of infected B. megatherium cells which develop lysogenic colonies depends on the number and kind of infecting virus particles and on the culture medium in which the cells are growing. (2) Cells infected with 100 or more T virus particles (from megatherium 899) in yeast extract peptone disintegrate, produce very few virus particles, and less than one lysogenic colony per 10(7) infected cells. Cells infected with one or a few particles produce 500 to 1000 virus particles each and about 30 lysogenic colonies per 10(7) infected colonies. (3) T phage obtained from lysogenic magatherium KM cultures produces many more lysogenic cells than does the original megatherium 899 virus. (4) Cells infected with megatherium 899 T virus in peptone medium and then transferred to asparagine medium give rise to 10(6) lysogenic colonies per 10(7) infected cells and this transformation will occur even after the infected cells have been in peptone for 60 to 90 minutes and are beginning to produce virus particles. (5) Continued growth of KM strain with either C or T virus from megatherium 899 for several hundred generations in the steady state apparatus results in a lysogenic strain which produces several different types of virus. |
format | Text |
id | pubmed-2195128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1961 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21951282008-04-23 Factors Controlling the Production of Lysogenic Cultures of B. megatherium Northrop, John H. J Gen Physiol Article (1) The proportion of infected B. megatherium cells which develop lysogenic colonies depends on the number and kind of infecting virus particles and on the culture medium in which the cells are growing. (2) Cells infected with 100 or more T virus particles (from megatherium 899) in yeast extract peptone disintegrate, produce very few virus particles, and less than one lysogenic colony per 10(7) infected cells. Cells infected with one or a few particles produce 500 to 1000 virus particles each and about 30 lysogenic colonies per 10(7) infected colonies. (3) T phage obtained from lysogenic magatherium KM cultures produces many more lysogenic cells than does the original megatherium 899 virus. (4) Cells infected with megatherium 899 T virus in peptone medium and then transferred to asparagine medium give rise to 10(6) lysogenic colonies per 10(7) infected cells and this transformation will occur even after the infected cells have been in peptone for 60 to 90 minutes and are beginning to produce virus particles. (5) Continued growth of KM strain with either C or T virus from megatherium 899 for several hundred generations in the steady state apparatus results in a lysogenic strain which produces several different types of virus. The Rockefeller University Press 1961-05-01 /pmc/articles/PMC2195128/ /pubmed/13729631 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Northrop, John H. Factors Controlling the Production of Lysogenic Cultures of B. megatherium |
title | Factors Controlling the Production of Lysogenic Cultures of B. megatherium
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title_full | Factors Controlling the Production of Lysogenic Cultures of B. megatherium
|
title_fullStr | Factors Controlling the Production of Lysogenic Cultures of B. megatherium
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title_full_unstemmed | Factors Controlling the Production of Lysogenic Cultures of B. megatherium
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title_short | Factors Controlling the Production of Lysogenic Cultures of B. megatherium
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title_sort | factors controlling the production of lysogenic cultures of b. megatherium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195128/ https://www.ncbi.nlm.nih.gov/pubmed/13729631 |
work_keys_str_mv | AT northropjohnh factorscontrollingtheproductionoflysogenicculturesofbmegatherium AT factorscontrollingtheproductionoflysogenicculturesofbmegatherium |