Studies on the Bohr Effect of Sheep Hemoglobin

A simple method is described for the measurement of pH changes in hemoglobin solutions on oxygenation and reduction. Data are presented establishing the absence of a Bohr effect in p-chloromercuribenzoate [PCMB] treated hemoglobin. The influence of a number of sulfhydryl inhibitors on the Bohr effec...

Descripción completa

Detalles Bibliográficos
Autores principales: Smyth, Derek G., Battaglia, Frederick C., Meschia, Giacomo
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195134/
https://www.ncbi.nlm.nih.gov/pubmed/19873538
_version_ 1782147774408556544
author Smyth, Derek G.
Battaglia, Frederick C.
Meschia, Giacomo
author_facet Smyth, Derek G.
Battaglia, Frederick C.
Meschia, Giacomo
author_sort Smyth, Derek G.
collection PubMed
description A simple method is described for the measurement of pH changes in hemoglobin solutions on oxygenation and reduction. Data are presented establishing the absence of a Bohr effect in p-chloromercuribenzoate [PCMB] treated hemoglobin. The influence of a number of sulfhydryl inhibitors on the Bohr effect of a hemoglobin solution is reported and an interpretation based on steric factors in the protein is proposed.
format Text
id pubmed-2195134
institution National Center for Biotechnology Information
language English
publishDate 1961
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21951342008-04-23 Studies on the Bohr Effect of Sheep Hemoglobin Smyth, Derek G. Battaglia, Frederick C. Meschia, Giacomo J Gen Physiol Article A simple method is described for the measurement of pH changes in hemoglobin solutions on oxygenation and reduction. Data are presented establishing the absence of a Bohr effect in p-chloromercuribenzoate [PCMB] treated hemoglobin. The influence of a number of sulfhydryl inhibitors on the Bohr effect of a hemoglobin solution is reported and an interpretation based on steric factors in the protein is proposed. The Rockefeller University Press 1961-05-01 /pmc/articles/PMC2195134/ /pubmed/19873538 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Smyth, Derek G.
Battaglia, Frederick C.
Meschia, Giacomo
Studies on the Bohr Effect of Sheep Hemoglobin
title Studies on the Bohr Effect of Sheep Hemoglobin
title_full Studies on the Bohr Effect of Sheep Hemoglobin
title_fullStr Studies on the Bohr Effect of Sheep Hemoglobin
title_full_unstemmed Studies on the Bohr Effect of Sheep Hemoglobin
title_short Studies on the Bohr Effect of Sheep Hemoglobin
title_sort studies on the bohr effect of sheep hemoglobin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195134/
https://www.ncbi.nlm.nih.gov/pubmed/19873538
work_keys_str_mv AT smythderekg studiesonthebohreffectofsheephemoglobin
AT battagliafrederickc studiesonthebohreffectofsheephemoglobin
AT meschiagiacomo studiesonthebohreffectofsheephemoglobin

Ejemplares similares