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Calcium Flux and Contractility in Guinea Pig Atria
The calcium in guinea pig atria can be divided into three components by kinetic studies with Ca(45): (a) a rapidly exchangeable fraction with a half-time of 4.5 minutes; (b) a slowly exchangeable fraction with a half-time of 86 (or 168) minutes; and (c) an inexchangeable fraction. In Krebs-Henseleit...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1962
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195178/ https://www.ncbi.nlm.nih.gov/pubmed/14007471 |
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author | Winegrad, Saul Shanes, Abraham M. |
author_facet | Winegrad, Saul Shanes, Abraham M. |
author_sort | Winegrad, Saul |
collection | PubMed |
description | The calcium in guinea pig atria can be divided into three components by kinetic studies with Ca(45): (a) a rapidly exchangeable fraction with a half-time of 4.5 minutes; (b) a slowly exchangeable fraction with a half-time of 86 (or 168) minutes; and (c) an inexchangeable fraction. In Krebs-Henseleit solution containing 2.5 mM calcium, the calcium content of the tissue at rest remains constant, the flux being about 0.02 µµmol/cm(2)-second. An increase or a decrease in extracellular calcium concentration by 1.25 mM causes a proportionate change in influx. A large increase in Ca(45) entry, equivalent to as much as 0.55 µµ/mol/cm(2) accompanies a contraction. When the strength of contraction is varied by stimulating at different frequencies or in solutions containing calcium at different concentrations, the increment of Ca(45) uptake per beat changes proportionally with the strength of the beat. Total atrial calcium is not increased by stimulation; however, the increase in outflux of Ca(45) during contraction that this constant tissue calcium implies could not be demonstrated under the experimental conditions employed. The observations are discussed in the light of the possible role of calcium transfer in excitation-contraction coupling. |
format | Text |
id | pubmed-2195178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1962 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21951782008-04-23 Calcium Flux and Contractility in Guinea Pig Atria Winegrad, Saul Shanes, Abraham M. J Gen Physiol Article The calcium in guinea pig atria can be divided into three components by kinetic studies with Ca(45): (a) a rapidly exchangeable fraction with a half-time of 4.5 minutes; (b) a slowly exchangeable fraction with a half-time of 86 (or 168) minutes; and (c) an inexchangeable fraction. In Krebs-Henseleit solution containing 2.5 mM calcium, the calcium content of the tissue at rest remains constant, the flux being about 0.02 µµmol/cm(2)-second. An increase or a decrease in extracellular calcium concentration by 1.25 mM causes a proportionate change in influx. A large increase in Ca(45) entry, equivalent to as much as 0.55 µµ/mol/cm(2) accompanies a contraction. When the strength of contraction is varied by stimulating at different frequencies or in solutions containing calcium at different concentrations, the increment of Ca(45) uptake per beat changes proportionally with the strength of the beat. Total atrial calcium is not increased by stimulation; however, the increase in outflux of Ca(45) during contraction that this constant tissue calcium implies could not be demonstrated under the experimental conditions employed. The observations are discussed in the light of the possible role of calcium transfer in excitation-contraction coupling. The Rockefeller University Press 1962-01-01 /pmc/articles/PMC2195178/ /pubmed/14007471 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Winegrad, Saul Shanes, Abraham M. Calcium Flux and Contractility in Guinea Pig Atria |
title | Calcium Flux and Contractility in Guinea Pig Atria |
title_full | Calcium Flux and Contractility in Guinea Pig Atria |
title_fullStr | Calcium Flux and Contractility in Guinea Pig Atria |
title_full_unstemmed | Calcium Flux and Contractility in Guinea Pig Atria |
title_short | Calcium Flux and Contractility in Guinea Pig Atria |
title_sort | calcium flux and contractility in guinea pig atria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195178/ https://www.ncbi.nlm.nih.gov/pubmed/14007471 |
work_keys_str_mv | AT winegradsaul calciumfluxandcontractilityinguineapigatria AT shanesabrahamm calciumfluxandcontractilityinguineapigatria |