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Heritability and Control of Differentiated Function in Cultured Cells
An established tissue culture cell line which retains a differentiated function in vitro is described. The cell line is of connective tissue origin, and its characteristic property is the synthesis and secretion of acid mucopolysaccharides, mainly hyaluronic acid. This differentiated cell function,...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1963
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195304/ https://www.ncbi.nlm.nih.gov/pubmed/14025353 |
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author | Davidson, E. H. |
author_facet | Davidson, E. H. |
author_sort | Davidson, E. H. |
collection | PubMed |
description | An established tissue culture cell line which retains a differentiated function in vitro is described. The cell line is of connective tissue origin, and its characteristic property is the synthesis and secretion of acid mucopolysaccharides, mainly hyaluronic acid. This differentiated cell function, the activity of which depends on continuous gene action, was found to be possessed by each of eleven clonal substrains, and is therefore a genetically heritable cell character. Rate of acid mucopolysaccharide biosynthesis falls sharply under the influence of the environmental conditions existing in crowded cultures, and this rate also declines if protein synthesis is directly inhibited with puromycin. Environmental modification of a differentiated product of gene action is thus illustrated in this study. |
format | Text |
id | pubmed-2195304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1963 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21953042008-04-23 Heritability and Control of Differentiated Function in Cultured Cells Davidson, E. H. J Gen Physiol Article An established tissue culture cell line which retains a differentiated function in vitro is described. The cell line is of connective tissue origin, and its characteristic property is the synthesis and secretion of acid mucopolysaccharides, mainly hyaluronic acid. This differentiated cell function, the activity of which depends on continuous gene action, was found to be possessed by each of eleven clonal substrains, and is therefore a genetically heritable cell character. Rate of acid mucopolysaccharide biosynthesis falls sharply under the influence of the environmental conditions existing in crowded cultures, and this rate also declines if protein synthesis is directly inhibited with puromycin. Environmental modification of a differentiated product of gene action is thus illustrated in this study. The Rockefeller University Press 1963-05-01 /pmc/articles/PMC2195304/ /pubmed/14025353 Text en Copyright © Copyright, 1963, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Davidson, E. H. Heritability and Control of Differentiated Function in Cultured Cells |
title | Heritability and Control of Differentiated Function in Cultured Cells |
title_full | Heritability and Control of Differentiated Function in Cultured Cells |
title_fullStr | Heritability and Control of Differentiated Function in Cultured Cells |
title_full_unstemmed | Heritability and Control of Differentiated Function in Cultured Cells |
title_short | Heritability and Control of Differentiated Function in Cultured Cells |
title_sort | heritability and control of differentiated function in cultured cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195304/ https://www.ncbi.nlm.nih.gov/pubmed/14025353 |
work_keys_str_mv | AT davidsoneh heritabilityandcontrolofdifferentiatedfunctioninculturedcells |