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Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain
The concentrations of the components of the respiratory chain were determined in a variety of intact skeletal muscles by a method of spectrophotometric observation of the transmitted light. In the case of the toad sartorius, these measurements were checked against isolated mitochondrial suspensions...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1963
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195307/ https://www.ncbi.nlm.nih.gov/pubmed/14041948 |
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author | Jöbsis, Frans F. |
author_facet | Jöbsis, Frans F. |
author_sort | Jöbsis, Frans F. |
collection | PubMed |
description | The concentrations of the components of the respiratory chain were determined in a variety of intact skeletal muscles by a method of spectrophotometric observation of the transmitted light. In the case of the toad sartorius, these measurements were checked against isolated mitochondrial suspensions prepared from toad skeletal muscles. The relative concentrations of the respiratory components were found to be in reasonable agreement with those of various mitochondrial preparations of mammalian tissues and of the ones from toad skeletal muscle. The rather low cytochrome b and pyridine nucleotide levels in the anoxic minus oxygenated difference spectra were shown to be caused to a certain degree by a partial reduction during the resting steady state. Upon treatment with a strong, reducing agent or after long anoxia some absorption bands appeared with maxima at 591, 562 to 564, and 432 to 434 mµ both in the intact and in the mitochondrial fractions of muscle tissue; they do not appear to be associated with the respiratory chain. |
format | Text |
id | pubmed-2195307 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1963 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21953072008-04-23 Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain Jöbsis, Frans F. J Gen Physiol Article The concentrations of the components of the respiratory chain were determined in a variety of intact skeletal muscles by a method of spectrophotometric observation of the transmitted light. In the case of the toad sartorius, these measurements were checked against isolated mitochondrial suspensions prepared from toad skeletal muscles. The relative concentrations of the respiratory components were found to be in reasonable agreement with those of various mitochondrial preparations of mammalian tissues and of the ones from toad skeletal muscle. The rather low cytochrome b and pyridine nucleotide levels in the anoxic minus oxygenated difference spectra were shown to be caused to a certain degree by a partial reduction during the resting steady state. Upon treatment with a strong, reducing agent or after long anoxia some absorption bands appeared with maxima at 591, 562 to 564, and 432 to 434 mµ both in the intact and in the mitochondrial fractions of muscle tissue; they do not appear to be associated with the respiratory chain. The Rockefeller University Press 1963-05-01 /pmc/articles/PMC2195307/ /pubmed/14041948 Text en Copyright © Copyright, 1963, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Jöbsis, Frans F. Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain |
title | Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain |
title_full | Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain |
title_fullStr | Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain |
title_full_unstemmed | Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain |
title_short | Spectrophotometric Studies on Intact Muscle : I. Components of the respiratory chain |
title_sort | spectrophotometric studies on intact muscle : i. components of the respiratory chain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195307/ https://www.ncbi.nlm.nih.gov/pubmed/14041948 |
work_keys_str_mv | AT jobsisfransf spectrophotometricstudiesonintactmuscleicomponentsoftherespiratorychain |