The Nature of the Muscle-Relaxing Factor : I. An improved assay system

Analysis of the effects of deoxycholate and benzalkonium chloride on granule relaxing activity, myofibril and glycerinated fiber responses to ATP, and the sensitivity of these actomyosin-containing systems to relaxing factors have established that it is possible to differentially inactivate intrinsi...

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Detalles Bibliográficos
Autores principales: Briggs, F. Norman, Fuchs, Franklin
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195309/
https://www.ncbi.nlm.nih.gov/pubmed/14015442
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author Briggs, F. Norman
Fuchs, Franklin
author_facet Briggs, F. Norman
Fuchs, Franklin
author_sort Briggs, F. Norman
collection PubMed
description Analysis of the effects of deoxycholate and benzalkonium chloride on granule relaxing activity, myofibril and glycerinated fiber responses to ATP, and the sensitivity of these actomyosin-containing systems to relaxing factors have established that it is possible to differentially inactivate intrinsic granule relaxing factor. It is therefore possible to use these agents to treat myofibrils and glycerinated muscle fibers so that they may be used for the assay of muscle-relaxing factor preparations. The destruction of intrinsic relaxing factor can be produced by treating actomyosin with 1 mM deoxycholate or 0.005 per cent benzalkonium chloride for 5 minutes at room temperature.
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spelling pubmed-21953092008-04-23 The Nature of the Muscle-Relaxing Factor : I. An improved assay system Briggs, F. Norman Fuchs, Franklin J Gen Physiol Article Analysis of the effects of deoxycholate and benzalkonium chloride on granule relaxing activity, myofibril and glycerinated fiber responses to ATP, and the sensitivity of these actomyosin-containing systems to relaxing factors have established that it is possible to differentially inactivate intrinsic granule relaxing factor. It is therefore possible to use these agents to treat myofibrils and glycerinated muscle fibers so that they may be used for the assay of muscle-relaxing factor preparations. The destruction of intrinsic relaxing factor can be produced by treating actomyosin with 1 mM deoxycholate or 0.005 per cent benzalkonium chloride for 5 minutes at room temperature. The Rockefeller University Press 1963-05-01 /pmc/articles/PMC2195309/ /pubmed/14015442 Text en Copyright © Copyright, 1963, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Briggs, F. Norman
Fuchs, Franklin
The Nature of the Muscle-Relaxing Factor : I. An improved assay system
title The Nature of the Muscle-Relaxing Factor : I. An improved assay system
title_full The Nature of the Muscle-Relaxing Factor : I. An improved assay system
title_fullStr The Nature of the Muscle-Relaxing Factor : I. An improved assay system
title_full_unstemmed The Nature of the Muscle-Relaxing Factor : I. An improved assay system
title_short The Nature of the Muscle-Relaxing Factor : I. An improved assay system
title_sort nature of the muscle-relaxing factor : i. an improved assay system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195309/
https://www.ncbi.nlm.nih.gov/pubmed/14015442
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