Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode

Electrical potential and resistance were measured with microelectrodes in in situ and isolated nuclei of gland cells of Drosophila flavorepleta. The nucleus-cytoplasm boundary was found to be rather impermeable to ion diffusion. It presents a resistance of the order of 1 Ω cm(2) and sustains a "...

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Autores principales: Loewenstein, Werner R., Kanno, Yoshinobu
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195317/
https://www.ncbi.nlm.nih.gov/pubmed/14042995
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author Loewenstein, Werner R.
Kanno, Yoshinobu
author_facet Loewenstein, Werner R.
Kanno, Yoshinobu
author_sort Loewenstein, Werner R.
collection PubMed
description Electrical potential and resistance were measured with microelectrodes in in situ and isolated nuclei of gland cells of Drosophila flavorepleta. The nucleus-cytoplasm boundary was found to be rather impermeable to ion diffusion. It presents a resistance of the order of 1 Ω cm(2) and sustains a "resting" potential, the nucleoplasm being about 15 mv negative with respect to the cytoplasm. Both the resistance and potential appear to be associated with the nuclear membrane: the potential declines to zero and the resistance to a fraction of its original value, when the membrane is perforated experimentally.
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spelling pubmed-21953172008-04-23 Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode Loewenstein, Werner R. Kanno, Yoshinobu J Gen Physiol Article Electrical potential and resistance were measured with microelectrodes in in situ and isolated nuclei of gland cells of Drosophila flavorepleta. The nucleus-cytoplasm boundary was found to be rather impermeable to ion diffusion. It presents a resistance of the order of 1 Ω cm(2) and sustains a "resting" potential, the nucleoplasm being about 15 mv negative with respect to the cytoplasm. Both the resistance and potential appear to be associated with the nuclear membrane: the potential declines to zero and the resistance to a fraction of its original value, when the membrane is perforated experimentally. The Rockefeller University Press 1963-07-01 /pmc/articles/PMC2195317/ /pubmed/14042995 Text en Copyright © Copyright, 1963, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Loewenstein, Werner R.
Kanno, Yoshinobu
Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode
title Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode
title_full Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode
title_fullStr Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode
title_full_unstemmed Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode
title_short Some Electrical Properties of a Nuclear Membrane Examined with a Microelectrode
title_sort some electrical properties of a nuclear membrane examined with a microelectrode
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195317/
https://www.ncbi.nlm.nih.gov/pubmed/14042995
work_keys_str_mv AT loewensteinwernerr someelectricalpropertiesofanuclearmembraneexaminedwithamicroelectrode
AT kannoyoshinobu someelectricalpropertiesofanuclearmembraneexaminedwithamicroelectrode

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