Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae

Partial recovery of ultraviolet-damaged denatured or native transforming DNA from Hemophilus influenzae, has been obtained by exposing the irradiated DNA in the denatured form to nitrous acid. Some factors that affect this recovery are described. An erythromycin marker (E(20)) was not reactivated. T...

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Autor principal: Cabrera-Juárez, Emiliano
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195342/
https://www.ncbi.nlm.nih.gov/pubmed/14080816
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author Cabrera-Juárez, Emiliano
author_facet Cabrera-Juárez, Emiliano
author_sort Cabrera-Juárez, Emiliano
collection PubMed
description Partial recovery of ultraviolet-damaged denatured or native transforming DNA from Hemophilus influenzae, has been obtained by exposing the irradiated DNA in the denatured form to nitrous acid. Some factors that affect this recovery are described. An erythromycin marker (E(20)) was not reactivated. The UV damage reactivable by nitrous acid is different from that repaired by the photoreactivating enzyme from bakers' yeast. The pretreatment with nitrous acid affords a slight protection for denatured C(25) DNA and Sm(250) DNA against ultraviolet irradiation, but this pretreatment sensitized the E(20) DNA to this irradiation.
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spelling pubmed-21953422008-04-23 Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae Cabrera-Juárez, Emiliano J Gen Physiol Article Partial recovery of ultraviolet-damaged denatured or native transforming DNA from Hemophilus influenzae, has been obtained by exposing the irradiated DNA in the denatured form to nitrous acid. Some factors that affect this recovery are described. An erythromycin marker (E(20)) was not reactivated. The UV damage reactivable by nitrous acid is different from that repaired by the photoreactivating enzyme from bakers' yeast. The pretreatment with nitrous acid affords a slight protection for denatured C(25) DNA and Sm(250) DNA against ultraviolet irradiation, but this pretreatment sensitized the E(20) DNA to this irradiation. The Rockefeller University Press 1963-11-01 /pmc/articles/PMC2195342/ /pubmed/14080816 Text en Copyright ©, 1964, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Cabrera-Juárez, Emiliano
Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae
title Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae
title_full Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae
title_fullStr Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae
title_full_unstemmed Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae
title_short Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae
title_sort nitrous acid reactivation of ultraviolet-irradiated transforming dna from hemophilus influenzae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195342/
https://www.ncbi.nlm.nih.gov/pubmed/14080816
work_keys_str_mv AT cabrerajuarezemiliano nitrousacidreactivationofultravioletirradiatedtransformingdnafromhemophilusinfluenzae

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