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Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder
Isolated urinary bladders of the bullfrog (R. catesbeiana) and the toad (B. marinus) were mounted in an Ussing chamber. Potential differences up to 114 mv were observed in bullfrog bladder when the mucosal surface was bathed in dilute Na(2)SO(4) and the serosal surface in sulfate Ringer's. In e...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1965
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195420/ https://www.ncbi.nlm.nih.gov/pubmed/14284782 |
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author | Leb, Daniel E. Hoshiko, T. |
author_facet | Leb, Daniel E. Hoshiko, T. |
author_sort | Leb, Daniel E. |
collection | PubMed |
description | Isolated urinary bladders of the bullfrog (R. catesbeiana) and the toad (B. marinus) were mounted in an Ussing chamber. Potential differences up to 114 mv were observed in bullfrog bladder when the mucosal surface was bathed in dilute Na(2)SO(4) and the serosal surface in sulfate Ringer's. In experiments with bullfrogs, K was used to replace Na in the mucosal solution and Na was used for K in the serosal solutions. The selectivity was judged in terms of the relative effectiveness of the replacement cation in maintaining the bladder potential. In experiments with toads, K and Rb were equally poor replacements for Na at the mucosal border, while Rb was a good replacement for K at the serosal border. Li in the mucosal solution appeared to depress the potential in part irreversibly. At the serosal border, Li was a partially effective substitute for K, more so than was Na. However, both were poor replacements compared to Rb. The mucosal surface of the urinary bladder of both frog and toad appears to be Na-selective and the serosal surface appears to be K-selective, consistent with the Koefoed-Johnsen-Ussing model for frog skin. |
format | Text |
id | pubmed-2195420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1965 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21954202008-04-23 Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder Leb, Daniel E. Hoshiko, T. J Gen Physiol Article Isolated urinary bladders of the bullfrog (R. catesbeiana) and the toad (B. marinus) were mounted in an Ussing chamber. Potential differences up to 114 mv were observed in bullfrog bladder when the mucosal surface was bathed in dilute Na(2)SO(4) and the serosal surface in sulfate Ringer's. In experiments with bullfrogs, K was used to replace Na in the mucosal solution and Na was used for K in the serosal solutions. The selectivity was judged in terms of the relative effectiveness of the replacement cation in maintaining the bladder potential. In experiments with toads, K and Rb were equally poor replacements for Na at the mucosal border, while Rb was a good replacement for K at the serosal border. Li in the mucosal solution appeared to depress the potential in part irreversibly. At the serosal border, Li was a partially effective substitute for K, more so than was Na. However, both were poor replacements compared to Rb. The mucosal surface of the urinary bladder of both frog and toad appears to be Na-selective and the serosal surface appears to be K-selective, consistent with the Koefoed-Johnsen-Ussing model for frog skin. The Rockefeller University Press 1965-01-01 /pmc/articles/PMC2195420/ /pubmed/14284782 Text en Copyright © 1965 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Leb, Daniel E. Hoshiko, T. Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder |
title | Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder |
title_full | Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder |
title_fullStr | Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder |
title_full_unstemmed | Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder |
title_short | Effects of Alkali Metal Gations on the Potential across Toad and Bullfrog Urinary Bladder |
title_sort | effects of alkali metal gations on the potential across toad and bullfrog urinary bladder |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195420/ https://www.ncbi.nlm.nih.gov/pubmed/14284782 |
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