Cargando…
Intracellular Electrical Potentials in Frog Skin
The influence of changes in ionic composition of the bathing solutions on intracellular electrical potentials in frog skin has been examined. When the skin bathed in SO(4) Ringer's solution is penetrated with a microelectrode two approximately equal potential jumps were frequently observed and...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1965
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195432/ https://www.ncbi.nlm.nih.gov/pubmed/14324974 |
_version_ | 1782147844207017984 |
---|---|
author | Cereijido, Marcelino Curran, Peter F. |
author_facet | Cereijido, Marcelino Curran, Peter F. |
author_sort | Cereijido, Marcelino |
collection | PubMed |
description | The influence of changes in ionic composition of the bathing solutions on intracellular electrical potentials in frog skin has been examined. When the skin bathed in SO(4) Ringer's solution is penetrated with a microelectrode two approximately equal potential jumps were frequently observed and most experiments were carried out with the electrode located between these steps. Substitution of Cl for SO(4) in the bathing solutions caused a decrease in PD across both the "outer" and "inner" barriers. When the skin was short-circuited an average intracellular potential of -18 mv was found with both Cl and SO(4) Ringer's. With the skin in SO4 Ringer's, decrease in Na concentration of the outside solution caused a decrease in PD between the microelectrode and the outside solution which was approximately the same as the decrease in total skin PD. With SO(4) Ringer's, an increase in K concentration in the inside solution caused a marked decrease in total skin PD. However, only 50 per cent of this change occurred at the inner barrier, between the microelectrode and the inside solution. The remainder of the change occurred at the outer barrier. This observation does not appear to be consistent with the model of the skin proposed by Koefoed-Johnson and Ussing (Acta Physiol. Scand., 1958, 42, 298). |
format | Text |
id | pubmed-2195432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1965 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21954322008-04-23 Intracellular Electrical Potentials in Frog Skin Cereijido, Marcelino Curran, Peter F. J Gen Physiol Article The influence of changes in ionic composition of the bathing solutions on intracellular electrical potentials in frog skin has been examined. When the skin bathed in SO(4) Ringer's solution is penetrated with a microelectrode two approximately equal potential jumps were frequently observed and most experiments were carried out with the electrode located between these steps. Substitution of Cl for SO(4) in the bathing solutions caused a decrease in PD across both the "outer" and "inner" barriers. When the skin was short-circuited an average intracellular potential of -18 mv was found with both Cl and SO(4) Ringer's. With the skin in SO4 Ringer's, decrease in Na concentration of the outside solution caused a decrease in PD between the microelectrode and the outside solution which was approximately the same as the decrease in total skin PD. With SO(4) Ringer's, an increase in K concentration in the inside solution caused a marked decrease in total skin PD. However, only 50 per cent of this change occurred at the inner barrier, between the microelectrode and the inside solution. The remainder of the change occurred at the outer barrier. This observation does not appear to be consistent with the model of the skin proposed by Koefoed-Johnson and Ussing (Acta Physiol. Scand., 1958, 42, 298). The Rockefeller University Press 1965-03-01 /pmc/articles/PMC2195432/ /pubmed/14324974 Text en Copyright © 1965 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Cereijido, Marcelino Curran, Peter F. Intracellular Electrical Potentials in Frog Skin |
title | Intracellular Electrical Potentials in Frog Skin |
title_full | Intracellular Electrical Potentials in Frog Skin |
title_fullStr | Intracellular Electrical Potentials in Frog Skin |
title_full_unstemmed | Intracellular Electrical Potentials in Frog Skin |
title_short | Intracellular Electrical Potentials in Frog Skin |
title_sort | intracellular electrical potentials in frog skin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195432/ https://www.ncbi.nlm.nih.gov/pubmed/14324974 |
work_keys_str_mv | AT cereijidomarcelino intracellularelectricalpotentialsinfrogskin AT curranpeterf intracellularelectricalpotentialsinfrogskin |