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Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer
Under the voltage clamp condition, the K inactivation was analyzed in cells bathed in the isosmotic KCl Lophius-Ringer solution. After conditioning hyperpolarization, the cells respond to depolarizations with increased K permeability, which in turn is decreased during maintained depolarizations. The...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1966
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195516/ https://www.ncbi.nlm.nih.gov/pubmed/5943605 |
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author | Nakajima, Shigehiro |
author_facet | Nakajima, Shigehiro |
author_sort | Nakajima, Shigehiro |
collection | PubMed |
description | Under the voltage clamp condition, the K inactivation was analyzed in cells bathed in the isosmotic KCl Lophius-Ringer solution. After conditioning hyperpolarization, the cells respond to depolarizations with increased K permeability, which in turn is decreased during maintained depolarizations. The steady-state levels of the K inactivation as a function of the membrane potential are related by an S-shaped curve similar to that which describes the steady-state Na inactivation in the squid giant axon. TEA reduced the K conductance by a factor which is independent of the potential, and without a shift of the inactivation curve along the voltage axis. The rapid phase of the K activation is less susceptible to TEA than the slow phase of the K activation. Hyperpolarizing steps remove the K inactivation, the rate of the removal being faster the larger the hyperpolarization from the standard potential of about -60 mv. |
format | Text |
id | pubmed-2195516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1966 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21955162008-04-23 Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer Nakajima, Shigehiro J Gen Physiol Article Under the voltage clamp condition, the K inactivation was analyzed in cells bathed in the isosmotic KCl Lophius-Ringer solution. After conditioning hyperpolarization, the cells respond to depolarizations with increased K permeability, which in turn is decreased during maintained depolarizations. The steady-state levels of the K inactivation as a function of the membrane potential are related by an S-shaped curve similar to that which describes the steady-state Na inactivation in the squid giant axon. TEA reduced the K conductance by a factor which is independent of the potential, and without a shift of the inactivation curve along the voltage axis. The rapid phase of the K activation is less susceptible to TEA than the slow phase of the K activation. Hyperpolarizing steps remove the K inactivation, the rate of the removal being faster the larger the hyperpolarization from the standard potential of about -60 mv. The Rockefeller University Press 1966-03-01 /pmc/articles/PMC2195516/ /pubmed/5943605 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Nakajima, Shigehiro Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer |
title | Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer |
title_full | Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer |
title_fullStr | Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer |
title_full_unstemmed | Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer |
title_short | Analysis of K Inactivation and TEA Action in the Supramedullary Cells of Puffer |
title_sort | analysis of k inactivation and tea action in the supramedullary cells of puffer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195516/ https://www.ncbi.nlm.nih.gov/pubmed/5943605 |
work_keys_str_mv | AT nakajimashigehiro analysisofkinactivationandteaactioninthesupramedullarycellsofpuffer |