Enzymatic Mechanisms of DNA Replication

DNA polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. Failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized DNA, described as nondenaturability and branching....

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Detalles Bibliográficos
Autores principales: Mitra, Sankar, Kornberg, Arthur
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
DNA and Alterations of DNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195533/
https://www.ncbi.nlm.nih.gov/pubmed/5338562
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author Mitra, Sankar
Kornberg, Arthur
author_facet Mitra, Sankar
Kornberg, Arthur
author_sort Mitra, Sankar
collection PubMed
description DNA polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. Failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized DNA, described as nondenaturability and branching. Aberrations in synthesized DNA were not observed when (a) the 5'-strand was destroyed by a specific nuclease during the course of replication or (b) a single-stranded (circular) phage (M13) DNA served as template. Replication of a single-stranded, circular DNA produced a helical product, but the nature of initiation of a new strand by the circular template remains to be explained. Hypothetical mechanisms for simultaneous replication of the 5'-strand are presented as is the possibility that the tertiary structure of the DNA, as for example, a circular form of the helix, is of prime importance in in vivo replication.
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spelling pubmed-21955332008-04-23 Enzymatic Mechanisms of DNA Replication Mitra, Sankar Kornberg, Arthur J Gen Physiol DNA and Alterations of DNA DNA polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. Failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized DNA, described as nondenaturability and branching. Aberrations in synthesized DNA were not observed when (a) the 5'-strand was destroyed by a specific nuclease during the course of replication or (b) a single-stranded (circular) phage (M13) DNA served as template. Replication of a single-stranded, circular DNA produced a helical product, but the nature of initiation of a new strand by the circular template remains to be explained. Hypothetical mechanisms for simultaneous replication of the 5'-strand are presented as is the possibility that the tertiary structure of the DNA, as for example, a circular form of the helix, is of prime importance in in vivo replication. The Rockefeller University Press 1966-07-01 /pmc/articles/PMC2195533/ /pubmed/5338562 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle DNA and Alterations of DNA
Mitra, Sankar
Kornberg, Arthur
Enzymatic Mechanisms of DNA Replication
title Enzymatic Mechanisms of DNA Replication
title_full Enzymatic Mechanisms of DNA Replication
title_fullStr Enzymatic Mechanisms of DNA Replication
title_full_unstemmed Enzymatic Mechanisms of DNA Replication
title_short Enzymatic Mechanisms of DNA Replication
title_sort enzymatic mechanisms of dna replication
topic DNA and Alterations of DNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195533/
https://www.ncbi.nlm.nih.gov/pubmed/5338562
work_keys_str_mv AT mitrasankar enzymaticmechanismsofdnareplication
AT kornbergarthur enzymaticmechanismsofdnareplication

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