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Genetic Studies of Recombining DNA in Pneumococcal Transformation

The results of genetic fine structure experiments, performed on the amiA locus of Pneumococcus are summarized. The peculiar feature of transformation genetics is that a given donor marker mutation transforms with an efficiency characteristic of the mutated site. In spite of this difficulty, mapping...

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Detalles Bibliográficos
Autores principales: Ephrussi-Taylor, Harriett, Gray, Thomas C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195543/
https://www.ncbi.nlm.nih.gov/pubmed/4381782
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author Ephrussi-Taylor, Harriett
Gray, Thomas C.
author_facet Ephrussi-Taylor, Harriett
Gray, Thomas C.
author_sort Ephrussi-Taylor, Harriett
collection PubMed
description The results of genetic fine structure experiments, performed on the amiA locus of Pneumococcus are summarized. The peculiar feature of transformation genetics is that a given donor marker mutation transforms with an efficiency characteristic of the mutated site. In spite of this difficulty, mapping procedures have been devised and quantitative recombination studies performed. It is concluded from these studies that transformation, in this locus, is the consequence of frequent, and essentially random exchanges occurring between donor DNA and the chromosomal DNA of the recipient cell. The average length of uninterrupted donor DNA polynucleotide strand which could be inserted into the chromosome of a transformed cell is estimated, from genetic data, to be probably not greater than 3·10(5) daltons (for a double-stranded insertion). It is proposed, on the basis of genetic evidence, that following essentially random exchanges between donor DNA and recipient chromosome, a revision process, specific for certain types of mutated sites, occurs. The revision process appears to remove preferentially donor DNA sequences from the primary recombinant structure, and allow repair along the chromosomal template, leading to low efficiency in the genetic integration of these sites. A mechanism for this "destruction-choice" process is presented, and evidence in support of this mechanism discussed.
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spelling pubmed-21955432008-04-23 Genetic Studies of Recombining DNA in Pneumococcal Transformation Ephrussi-Taylor, Harriett Gray, Thomas C. J Gen Physiol Genetic Transformation The results of genetic fine structure experiments, performed on the amiA locus of Pneumococcus are summarized. The peculiar feature of transformation genetics is that a given donor marker mutation transforms with an efficiency characteristic of the mutated site. In spite of this difficulty, mapping procedures have been devised and quantitative recombination studies performed. It is concluded from these studies that transformation, in this locus, is the consequence of frequent, and essentially random exchanges occurring between donor DNA and the chromosomal DNA of the recipient cell. The average length of uninterrupted donor DNA polynucleotide strand which could be inserted into the chromosome of a transformed cell is estimated, from genetic data, to be probably not greater than 3·10(5) daltons (for a double-stranded insertion). It is proposed, on the basis of genetic evidence, that following essentially random exchanges between donor DNA and recipient chromosome, a revision process, specific for certain types of mutated sites, occurs. The revision process appears to remove preferentially donor DNA sequences from the primary recombinant structure, and allow repair along the chromosomal template, leading to low efficiency in the genetic integration of these sites. A mechanism for this "destruction-choice" process is presented, and evidence in support of this mechanism discussed. The Rockefeller University Press 1966-07-01 /pmc/articles/PMC2195543/ /pubmed/4381782 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Genetic Transformation
Ephrussi-Taylor, Harriett
Gray, Thomas C.
Genetic Studies of Recombining DNA in Pneumococcal Transformation
title Genetic Studies of Recombining DNA in Pneumococcal Transformation
title_full Genetic Studies of Recombining DNA in Pneumococcal Transformation
title_fullStr Genetic Studies of Recombining DNA in Pneumococcal Transformation
title_full_unstemmed Genetic Studies of Recombining DNA in Pneumococcal Transformation
title_short Genetic Studies of Recombining DNA in Pneumococcal Transformation
title_sort genetic studies of recombining dna in pneumococcal transformation
topic Genetic Transformation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195543/
https://www.ncbi.nlm.nih.gov/pubmed/4381782
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