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High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis
In a screen designed to identify genes that regulate T cell receptor (TCR)/CD3-mediated apoptosis, we found that high level expression of CD43 protected T cell hybridomas from activation-induced cell death. The protection appears to result from its capacity to block Fas-mediated death signals rather...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195722/ https://www.ncbi.nlm.nih.gov/pubmed/10601365 |
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author | He, You-Wen Bevan, Michael J. |
author_facet | He, You-Wen Bevan, Michael J. |
author_sort | He, You-Wen |
collection | PubMed |
description | In a screen designed to identify genes that regulate T cell receptor (TCR)/CD3-mediated apoptosis, we found that high level expression of CD43 protected T cell hybridomas from activation-induced cell death. The protection appears to result from its capacity to block Fas-mediated death signals rather than from inhibition of the upregulation of Fas and/or Fas ligand after T cell stimulation. We found that peripheral CD4(+) T cells can be divided into two subsets based on the level of CD43 surface expression. The CD4(+)CD43(low) subset exhibits a naive T cell phenotype, being CD62L(high)CD45RB(high)CD44(low), whereas CD4(+)CD43(high) cells exhibit a memory phenotype, being CD62L(low)CD45RB(low)CD44(high). Recent studies have demonstrated that engagement of TCR and Fas induces naive CD4(+) T cells to undergo apoptosis, and the same treatment enhances the proliferation of memory CD4(+) T cells. We confirm here that peripheral CD4(+)CD43(high) T cells are resistant to TCR/CD3-mediated cell death. These results suggest that the expression levels of CD43 on naive and memory CD4(+) T cells determine their susceptibility to Fas-dependent cell death and that high level expression of CD43 may be used as a marker to define CD4(+) memory T cells. Expression of CD43 provides a novel mechanism by which tumor cells expressing abnormally high levels of CD43 may escape Fas-mediated killing. |
format | Text |
id | pubmed-2195722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21957222008-04-16 High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis He, You-Wen Bevan, Michael J. J Exp Med Brief Definitive Report In a screen designed to identify genes that regulate T cell receptor (TCR)/CD3-mediated apoptosis, we found that high level expression of CD43 protected T cell hybridomas from activation-induced cell death. The protection appears to result from its capacity to block Fas-mediated death signals rather than from inhibition of the upregulation of Fas and/or Fas ligand after T cell stimulation. We found that peripheral CD4(+) T cells can be divided into two subsets based on the level of CD43 surface expression. The CD4(+)CD43(low) subset exhibits a naive T cell phenotype, being CD62L(high)CD45RB(high)CD44(low), whereas CD4(+)CD43(high) cells exhibit a memory phenotype, being CD62L(low)CD45RB(low)CD44(high). Recent studies have demonstrated that engagement of TCR and Fas induces naive CD4(+) T cells to undergo apoptosis, and the same treatment enhances the proliferation of memory CD4(+) T cells. We confirm here that peripheral CD4(+)CD43(high) T cells are resistant to TCR/CD3-mediated cell death. These results suggest that the expression levels of CD43 on naive and memory CD4(+) T cells determine their susceptibility to Fas-dependent cell death and that high level expression of CD43 may be used as a marker to define CD4(+) memory T cells. Expression of CD43 provides a novel mechanism by which tumor cells expressing abnormally high levels of CD43 may escape Fas-mediated killing. The Rockefeller University Press 1999-12-20 /pmc/articles/PMC2195722/ /pubmed/10601365 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Brief Definitive Report He, You-Wen Bevan, Michael J. High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis |
title | High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis |
title_full | High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis |
title_fullStr | High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis |
title_full_unstemmed | High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis |
title_short | High Level Expression of Cd43 Inhibits T Cell Receptor/CD3-Mediated Apoptosis |
title_sort | high level expression of cd43 inhibits t cell receptor/cd3-mediated apoptosis |
topic | Brief Definitive Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195722/ https://www.ncbi.nlm.nih.gov/pubmed/10601365 |
work_keys_str_mv | AT heyouwen highlevelexpressionofcd43inhibitstcellreceptorcd3mediatedapoptosis AT bevanmichaelj highlevelexpressionofcd43inhibitstcellreceptorcd3mediatedapoptosis |