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Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph

Murine gut intraepithelial (IEL) T cell receptor (TCR)-α/β(1) lymphocytes bearing CD8α/β or CD8α/α coreceptors have been shown previously to express different oligoclonal TCR β chain repertoires in the same mouse, in agreement with other evidence indicating that these two populations belong to diffe...

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Autores principales: Arstila, Tuula, Arstila, T. Petteri, Calbo, Sébastien, Selz, Françoise, Malassis-Seris, Michèle, Vassalli, Pierre, Kourilsky, Philippe, Guy-Grand, Delphine
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195856/
https://www.ncbi.nlm.nih.gov/pubmed/10755885
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author Arstila, Tuula
Arstila, T. Petteri
Calbo, Sébastien
Selz, Françoise
Malassis-Seris, Michèle
Vassalli, Pierre
Kourilsky, Philippe
Guy-Grand, Delphine
author_facet Arstila, Tuula
Arstila, T. Petteri
Calbo, Sébastien
Selz, Françoise
Malassis-Seris, Michèle
Vassalli, Pierre
Kourilsky, Philippe
Guy-Grand, Delphine
author_sort Arstila, Tuula
collection PubMed
description Murine gut intraepithelial (IEL) T cell receptor (TCR)-α/β(1) lymphocytes bearing CD8α/β or CD8α/α coreceptors have been shown previously to express different oligoclonal TCR β chain repertoires in the same mouse, in agreement with other evidence indicating that these two populations belong to different ontogenic lineages, with only CD8α/β(1) IELs being fully thymus dependent. CD8α/β(1), but not CD8α/α(1), T lymphocytes are also present in the lamina propria. Here, we show that CD8α/β(+) lymphocytes from the lamina propria and the epithelium are both oligoclonal, and that they share the same TCR-β clonotypes in the same mouse, as is also the case for CD4(+) T cells. Furthermore, identical T cell clones were detected among CD8α/β(1) IELs and CD8α/β(1) blasts circulating into the thoracic duct (TD) lymph of the same mouse, whereas TD small lymphocytes are polyclonal. These findings must be considered in light of previous observations showing that T blasts, but not small T lymphocytes, circulating in the TD lymph have the capacity of homing into the gut epithelium and lamina propria. These combined observations have interesting implications for our understanding of the recirculation of gut thymus-dependent lymphocytes and their precursors, and of the events leading up to the selection of their restricted TCR repertoire.
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spelling pubmed-21958562008-04-16 Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph Arstila, Tuula Arstila, T. Petteri Calbo, Sébastien Selz, Françoise Malassis-Seris, Michèle Vassalli, Pierre Kourilsky, Philippe Guy-Grand, Delphine J Exp Med Original Article Murine gut intraepithelial (IEL) T cell receptor (TCR)-α/β(1) lymphocytes bearing CD8α/β or CD8α/α coreceptors have been shown previously to express different oligoclonal TCR β chain repertoires in the same mouse, in agreement with other evidence indicating that these two populations belong to different ontogenic lineages, with only CD8α/β(1) IELs being fully thymus dependent. CD8α/β(1), but not CD8α/α(1), T lymphocytes are also present in the lamina propria. Here, we show that CD8α/β(+) lymphocytes from the lamina propria and the epithelium are both oligoclonal, and that they share the same TCR-β clonotypes in the same mouse, as is also the case for CD4(+) T cells. Furthermore, identical T cell clones were detected among CD8α/β(1) IELs and CD8α/β(1) blasts circulating into the thoracic duct (TD) lymph of the same mouse, whereas TD small lymphocytes are polyclonal. These findings must be considered in light of previous observations showing that T blasts, but not small T lymphocytes, circulating in the TD lymph have the capacity of homing into the gut epithelium and lamina propria. These combined observations have interesting implications for our understanding of the recirculation of gut thymus-dependent lymphocytes and their precursors, and of the events leading up to the selection of their restricted TCR repertoire. The Rockefeller University Press 2000-03-06 /pmc/articles/PMC2195856/ /pubmed/10755885 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Arstila, Tuula
Arstila, T. Petteri
Calbo, Sébastien
Selz, Françoise
Malassis-Seris, Michèle
Vassalli, Pierre
Kourilsky, Philippe
Guy-Grand, Delphine
Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph
title Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph
title_full Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph
title_fullStr Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph
title_full_unstemmed Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph
title_short Identical T Cell Clones Are Located within the Mouse Gut Epithelium and Lamina Propria and Circulate in the Thoracic Duct Lymph
title_sort identical t cell clones are located within the mouse gut epithelium and lamina propria and circulate in the thoracic duct lymph
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195856/
https://www.ncbi.nlm.nih.gov/pubmed/10755885
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