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Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice

C57BL/6 mice genetically deficient in interleukin 15 (IL-15(−/−) mice) were generated by gene targeting. IL-15(−/−) mice displayed marked reductions in numbers of thymic and peripheral natural killer (NK) T cells, memory phenotype CD8(+) T cells, and distinct subpopulations of intestinal intraepithe...

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Autores principales: Kennedy, Mary K., Glaccum, Moira, Brown, Sandra N., Butz, Eric A., Viney, Joanne L., Embers, Monica, Matsuki, Naoto, Charrier, Keith, Sedger, Lisa, Willis, Cynthia R., Brasel, Kenneth, Morrissey, Philip J., Stocking, Kim, Schuh, JoAnn C. L., Joyce, Sebastian, Peschon, Jacques J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195858/
https://www.ncbi.nlm.nih.gov/pubmed/10704459
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author Kennedy, Mary K.
Glaccum, Moira
Brown, Sandra N.
Butz, Eric A.
Viney, Joanne L.
Embers, Monica
Matsuki, Naoto
Charrier, Keith
Sedger, Lisa
Willis, Cynthia R.
Brasel, Kenneth
Morrissey, Philip J.
Stocking, Kim
Schuh, JoAnn C. L.
Joyce, Sebastian
Peschon, Jacques J.
author_facet Kennedy, Mary K.
Glaccum, Moira
Brown, Sandra N.
Butz, Eric A.
Viney, Joanne L.
Embers, Monica
Matsuki, Naoto
Charrier, Keith
Sedger, Lisa
Willis, Cynthia R.
Brasel, Kenneth
Morrissey, Philip J.
Stocking, Kim
Schuh, JoAnn C. L.
Joyce, Sebastian
Peschon, Jacques J.
author_sort Kennedy, Mary K.
collection PubMed
description C57BL/6 mice genetically deficient in interleukin 15 (IL-15(−/−) mice) were generated by gene targeting. IL-15(−/−) mice displayed marked reductions in numbers of thymic and peripheral natural killer (NK) T cells, memory phenotype CD8(+) T cells, and distinct subpopulations of intestinal intraepithelial lymphocytes (IELs). The reduction but not absence of these populations in IL-15(−/−) mice likely reflects an important role for IL-15 for expansion and/or survival of these cells. IL-15(−/−) mice lacked NK cells, as assessed by both immunophenotyping and functional criteria, indicating an obligate role for IL-15 in the development and functional maturation of NK cells. Specific defects associated with IL-15 deficiency were reversed by in vivo administration of exogenous IL-15. Despite their immunological defects, IL-15(−/−) mice remained healthy when maintained under specific pathogen-free conditions. However, IL-15(−/−) mice are likely to have compromised host defense responses to various pathogens, as they were unable to mount a protective response to challenge with vaccinia virus. These data reveal critical roles for IL-15 in the development of specific lymphoid lineages. Moreover, the ability to rescue lymphoid defects in IL-15(−/−) mice by IL-15 administration represents a powerful means by which to further elucidate the biological roles of this cytokine.
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spelling pubmed-21958582008-04-16 Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice Kennedy, Mary K. Glaccum, Moira Brown, Sandra N. Butz, Eric A. Viney, Joanne L. Embers, Monica Matsuki, Naoto Charrier, Keith Sedger, Lisa Willis, Cynthia R. Brasel, Kenneth Morrissey, Philip J. Stocking, Kim Schuh, JoAnn C. L. Joyce, Sebastian Peschon, Jacques J. J Exp Med Original Article C57BL/6 mice genetically deficient in interleukin 15 (IL-15(−/−) mice) were generated by gene targeting. IL-15(−/−) mice displayed marked reductions in numbers of thymic and peripheral natural killer (NK) T cells, memory phenotype CD8(+) T cells, and distinct subpopulations of intestinal intraepithelial lymphocytes (IELs). The reduction but not absence of these populations in IL-15(−/−) mice likely reflects an important role for IL-15 for expansion and/or survival of these cells. IL-15(−/−) mice lacked NK cells, as assessed by both immunophenotyping and functional criteria, indicating an obligate role for IL-15 in the development and functional maturation of NK cells. Specific defects associated with IL-15 deficiency were reversed by in vivo administration of exogenous IL-15. Despite their immunological defects, IL-15(−/−) mice remained healthy when maintained under specific pathogen-free conditions. However, IL-15(−/−) mice are likely to have compromised host defense responses to various pathogens, as they were unable to mount a protective response to challenge with vaccinia virus. These data reveal critical roles for IL-15 in the development of specific lymphoid lineages. Moreover, the ability to rescue lymphoid defects in IL-15(−/−) mice by IL-15 administration represents a powerful means by which to further elucidate the biological roles of this cytokine. The Rockefeller University Press 2000-03-06 /pmc/articles/PMC2195858/ /pubmed/10704459 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Kennedy, Mary K.
Glaccum, Moira
Brown, Sandra N.
Butz, Eric A.
Viney, Joanne L.
Embers, Monica
Matsuki, Naoto
Charrier, Keith
Sedger, Lisa
Willis, Cynthia R.
Brasel, Kenneth
Morrissey, Philip J.
Stocking, Kim
Schuh, JoAnn C. L.
Joyce, Sebastian
Peschon, Jacques J.
Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice
title Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice
title_full Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice
title_fullStr Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice
title_full_unstemmed Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice
title_short Reversible Defects in Natural Killer and Memory Cd8 T Cell Lineages in Interleukin 15–Deficient Mice
title_sort reversible defects in natural killer and memory cd8 t cell lineages in interleukin 15–deficient mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195858/
https://www.ncbi.nlm.nih.gov/pubmed/10704459
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