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Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9
MIHA is an inhibitor of apoptosis protein (IAP) that can inhibit cell death by direct interaction with caspases, the effector proteases of apoptosis. DIABLO is a mammalian protein that can bind to IAPs and antagonize their antiapoptotic effect, a function analogous to that of the proapoptotic Drosop...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195997/ https://www.ncbi.nlm.nih.gov/pubmed/11157976 |
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author | Ekert, Paul G. Silke, John Hawkins, Christine J. Verhagen, Anne M. Vaux, David L. |
author_facet | Ekert, Paul G. Silke, John Hawkins, Christine J. Verhagen, Anne M. Vaux, David L. |
author_sort | Ekert, Paul G. |
collection | PubMed |
description | MIHA is an inhibitor of apoptosis protein (IAP) that can inhibit cell death by direct interaction with caspases, the effector proteases of apoptosis. DIABLO is a mammalian protein that can bind to IAPs and antagonize their antiapoptotic effect, a function analogous to that of the proapoptotic Drosophila molecules, Grim, Reaper, and HID. Here, we show that after UV radiation, MIHA prevented apoptosis by inhibiting caspase 9 and caspase 3 activation. Unlike Bcl-2, MIHA functioned after release of cytochrome c and DIABLO from the mitochondria and was able to bind to both processed caspase 9 and processed caspase 3 to prevent feedback activation of their zymogen forms. Once released into the cytosol, DIABLO bound to MIHA and disrupted its association with processed caspase 9, thereby allowing caspase 9 to activate caspase 3, resulting in apoptosis. |
format | Text |
id | pubmed-2195997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21959972008-05-01 Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9 Ekert, Paul G. Silke, John Hawkins, Christine J. Verhagen, Anne M. Vaux, David L. J Cell Biol Original Article MIHA is an inhibitor of apoptosis protein (IAP) that can inhibit cell death by direct interaction with caspases, the effector proteases of apoptosis. DIABLO is a mammalian protein that can bind to IAPs and antagonize their antiapoptotic effect, a function analogous to that of the proapoptotic Drosophila molecules, Grim, Reaper, and HID. Here, we show that after UV radiation, MIHA prevented apoptosis by inhibiting caspase 9 and caspase 3 activation. Unlike Bcl-2, MIHA functioned after release of cytochrome c and DIABLO from the mitochondria and was able to bind to both processed caspase 9 and processed caspase 3 to prevent feedback activation of their zymogen forms. Once released into the cytosol, DIABLO bound to MIHA and disrupted its association with processed caspase 9, thereby allowing caspase 9 to activate caspase 3, resulting in apoptosis. The Rockefeller University Press 2001-02-05 /pmc/articles/PMC2195997/ /pubmed/11157976 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Ekert, Paul G. Silke, John Hawkins, Christine J. Verhagen, Anne M. Vaux, David L. Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9 |
title | Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9 |
title_full | Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9 |
title_fullStr | Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9 |
title_full_unstemmed | Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9 |
title_short | Diablo Promotes Apoptosis by Removing Miha/Xiap from Processed Caspase 9 |
title_sort | diablo promotes apoptosis by removing miha/xiap from processed caspase 9 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195997/ https://www.ncbi.nlm.nih.gov/pubmed/11157976 |
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