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Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendri...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196090/ https://www.ncbi.nlm.nih.gov/pubmed/12835480 http://dx.doi.org/10.1084/jem.20022230 |
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author | Hurez, Vincent Saparov, Arman Tousson, Albert Fuller, Michael J. Kubo, Takekazu Oliver, James Weaver, Benjamin T. Weaver, Casey T. |
author_facet | Hurez, Vincent Saparov, Arman Tousson, Albert Fuller, Michael J. Kubo, Takekazu Oliver, James Weaver, Benjamin T. Weaver, Casey T. |
author_sort | Hurez, Vincent |
collection | PubMed |
description | Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)–T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription. |
format | Text |
id | pubmed-2196090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21960902008-04-11 Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells Hurez, Vincent Saparov, Arman Tousson, Albert Fuller, Michael J. Kubo, Takekazu Oliver, James Weaver, Benjamin T. Weaver, Casey T. J Exp Med Article Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)–T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription. The Rockefeller University Press 2003-07-07 /pmc/articles/PMC2196090/ /pubmed/12835480 http://dx.doi.org/10.1084/jem.20022230 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Hurez, Vincent Saparov, Arman Tousson, Albert Fuller, Michael J. Kubo, Takekazu Oliver, James Weaver, Benjamin T. Weaver, Casey T. Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells |
title | Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells |
title_full | Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells |
title_fullStr | Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells |
title_full_unstemmed | Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells |
title_short | Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells |
title_sort | restricted clonal expression of il-2 by naive t cells reflects differential dynamic interactions with dendritic cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196090/ https://www.ncbi.nlm.nih.gov/pubmed/12835480 http://dx.doi.org/10.1084/jem.20022230 |
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