Cargando…

Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells

Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendri...

Descripción completa

Detalles Bibliográficos
Autores principales: Hurez, Vincent, Saparov, Arman, Tousson, Albert, Fuller, Michael J., Kubo, Takekazu, Oliver, James, Weaver, Benjamin T., Weaver, Casey T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196090/
https://www.ncbi.nlm.nih.gov/pubmed/12835480
http://dx.doi.org/10.1084/jem.20022230
_version_ 1782147994320109568
author Hurez, Vincent
Saparov, Arman
Tousson, Albert
Fuller, Michael J.
Kubo, Takekazu
Oliver, James
Weaver, Benjamin T.
Weaver, Casey T.
author_facet Hurez, Vincent
Saparov, Arman
Tousson, Albert
Fuller, Michael J.
Kubo, Takekazu
Oliver, James
Weaver, Benjamin T.
Weaver, Casey T.
author_sort Hurez, Vincent
collection PubMed
description Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)–T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription.
format Text
id pubmed-2196090
institution National Center for Biotechnology Information
language English
publishDate 2003
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21960902008-04-11 Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells Hurez, Vincent Saparov, Arman Tousson, Albert Fuller, Michael J. Kubo, Takekazu Oliver, James Weaver, Benjamin T. Weaver, Casey T. J Exp Med Article Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)–T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription. The Rockefeller University Press 2003-07-07 /pmc/articles/PMC2196090/ /pubmed/12835480 http://dx.doi.org/10.1084/jem.20022230 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hurez, Vincent
Saparov, Arman
Tousson, Albert
Fuller, Michael J.
Kubo, Takekazu
Oliver, James
Weaver, Benjamin T.
Weaver, Casey T.
Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
title Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
title_full Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
title_fullStr Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
title_full_unstemmed Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
title_short Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
title_sort restricted clonal expression of il-2 by naive t cells reflects differential dynamic interactions with dendritic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196090/
https://www.ncbi.nlm.nih.gov/pubmed/12835480
http://dx.doi.org/10.1084/jem.20022230
work_keys_str_mv AT hurezvincent restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells
AT saparovarman restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells
AT toussonalbert restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells
AT fullermichaelj restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells
AT kubotakekazu restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells
AT oliverjames restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells
AT weaverbenjamint restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells
AT weavercaseyt restrictedclonalexpressionofil2bynaivetcellsreflectsdifferentialdynamicinteractionswithdendriticcells