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rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells
Shigella, the causative agents of bacillary dysentery, are capable of invading mammalian cells that are not normally phagocytic. Uptake of bacteria by the mammalian cells is directed by bacterial factors named IpaB, IpaC, and IpaD invasins, in which Ipa invasins secreted into the bacterial environme...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196126/ https://www.ncbi.nlm.nih.gov/pubmed/9016877 |
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author | Watarai, Masahisa Kamata, Yoichi Kozaki, Shunji Sasakawa, Chihiro |
author_facet | Watarai, Masahisa Kamata, Yoichi Kozaki, Shunji Sasakawa, Chihiro |
author_sort | Watarai, Masahisa |
collection | PubMed |
description | Shigella, the causative agents of bacillary dysentery, are capable of invading mammalian cells that are not normally phagocytic. Uptake of bacteria by the mammalian cells is directed by bacterial factors named IpaB, IpaC, and IpaD invasins, in which Ipa invasins secreted into the bacterial environment can interact with α(5)β(1) integrin. We report here that Shigella invasion of epithelial cells requires rho activity, a ras-related GTP-binding protein. The invasive capacity of Shigella flexneri for Chinese hamster ovary (CHO) cells and other epithelial cells were greatly reduced when treated with Clostridium botulinum exoenzyme C3 transferase. Conversely, uptake of bacteria by CHO cells was promoted upon microinjection of an activated rho variant, Val14RhoA. Attachment of S. flexneri to CHO cells can elicit tyrosine phosphorylation of pp125(FAK) and paxillin, localized accumulation of F-actin, vinculin, and talin, and activation of protein kinase C, which were all blocked by the treatment with C3 transferase. Our results indicate that cellular signal transduction regulated by rho is essential for Shigella invasion of epithelial cells. |
format | Text |
id | pubmed-2196126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21961262008-04-16 rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells Watarai, Masahisa Kamata, Yoichi Kozaki, Shunji Sasakawa, Chihiro J Exp Med Article Shigella, the causative agents of bacillary dysentery, are capable of invading mammalian cells that are not normally phagocytic. Uptake of bacteria by the mammalian cells is directed by bacterial factors named IpaB, IpaC, and IpaD invasins, in which Ipa invasins secreted into the bacterial environment can interact with α(5)β(1) integrin. We report here that Shigella invasion of epithelial cells requires rho activity, a ras-related GTP-binding protein. The invasive capacity of Shigella flexneri for Chinese hamster ovary (CHO) cells and other epithelial cells were greatly reduced when treated with Clostridium botulinum exoenzyme C3 transferase. Conversely, uptake of bacteria by CHO cells was promoted upon microinjection of an activated rho variant, Val14RhoA. Attachment of S. flexneri to CHO cells can elicit tyrosine phosphorylation of pp125(FAK) and paxillin, localized accumulation of F-actin, vinculin, and talin, and activation of protein kinase C, which were all blocked by the treatment with C3 transferase. Our results indicate that cellular signal transduction regulated by rho is essential for Shigella invasion of epithelial cells. The Rockefeller University Press 1997-01-20 /pmc/articles/PMC2196126/ /pubmed/9016877 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Watarai, Masahisa Kamata, Yoichi Kozaki, Shunji Sasakawa, Chihiro rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells |
title | rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells |
title_full | rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells |
title_fullStr | rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells |
title_full_unstemmed | rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells |
title_short | rho, a Small GTP-Binding Protein, Is Essential for Shigella Invasion of Epithelial Cells |
title_sort | rho, a small gtp-binding protein, is essential for shigella invasion of epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196126/ https://www.ncbi.nlm.nih.gov/pubmed/9016877 |
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