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T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)
Lipopolysaccharide (LPS) from gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other APC. We show here that, under in vivo conditions, LPS also induces strong stimulation of T cells. As manifested by CD69 upregulation, LPS injection stimulates both CD...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196347/ https://www.ncbi.nlm.nih.gov/pubmed/9182680 |
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author | Tough, David F. Sun, Siquan Sprent, Jonathan |
author_facet | Tough, David F. Sun, Siquan Sprent, Jonathan |
author_sort | Tough, David F. |
collection | PubMed |
description | Lipopolysaccharide (LPS) from gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other APC. We show here that, under in vivo conditions, LPS also induces strong stimulation of T cells. As manifested by CD69 upregulation, LPS injection stimulates both CD4 and CD8(+) T cells, and, at high doses, stimulates naive (CD44(lo)) cells as well as memory (CD44(hi)) cells. However, in terms of cell division, the response of T cells after LPS injection is limited to the CD44(hi) subset of CD8(+) cells. In contrast with B cells, proliferative responses of CD44(hi) CD8(+) cells require only very low doses of LPS (10 ng). Based on studies with LPS-nonresponder and gene-knockout mice, LPS-induced proliferation of CD44(hi) CD8(+) cells appears to operate via an indirect pathway involving LPS stimulation of APC and release of type I (α, β) interferon (IFN-I). Similar selective stimulation of CD44(hi) CD8(+) cells occurs in viral infections and after injection of IFN-I, implying a common mechanism. Hence, intermittent exposure to pathogens (gram-negative bacteria and viruses) could contribute to the high background proliferation of memory–phenotype CD8(+) cells found in normal animals. |
format | Text |
id | pubmed-2196347 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21963472008-04-16 T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) Tough, David F. Sun, Siquan Sprent, Jonathan J Exp Med Article Lipopolysaccharide (LPS) from gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other APC. We show here that, under in vivo conditions, LPS also induces strong stimulation of T cells. As manifested by CD69 upregulation, LPS injection stimulates both CD4 and CD8(+) T cells, and, at high doses, stimulates naive (CD44(lo)) cells as well as memory (CD44(hi)) cells. However, in terms of cell division, the response of T cells after LPS injection is limited to the CD44(hi) subset of CD8(+) cells. In contrast with B cells, proliferative responses of CD44(hi) CD8(+) cells require only very low doses of LPS (10 ng). Based on studies with LPS-nonresponder and gene-knockout mice, LPS-induced proliferation of CD44(hi) CD8(+) cells appears to operate via an indirect pathway involving LPS stimulation of APC and release of type I (α, β) interferon (IFN-I). Similar selective stimulation of CD44(hi) CD8(+) cells occurs in viral infections and after injection of IFN-I, implying a common mechanism. Hence, intermittent exposure to pathogens (gram-negative bacteria and viruses) could contribute to the high background proliferation of memory–phenotype CD8(+) cells found in normal animals. The Rockefeller University Press 1997-06-16 /pmc/articles/PMC2196347/ /pubmed/9182680 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Tough, David F. Sun, Siquan Sprent, Jonathan T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) |
title | T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) |
title_full | T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) |
title_fullStr | T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) |
title_full_unstemmed | T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) |
title_short | T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) |
title_sort | t cell stimulation in vivo by lipopolysaccharide (lps) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196347/ https://www.ncbi.nlm.nih.gov/pubmed/9182680 |
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