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T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)

Lipopolysaccharide (LPS) from gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other APC. We show here that, under in vivo conditions, LPS also induces strong stimulation of T cells. As manifested by CD69 upregulation, LPS injection stimulates both CD...

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Detalles Bibliográficos
Autores principales: Tough, David F., Sun, Siquan, Sprent, Jonathan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196347/
https://www.ncbi.nlm.nih.gov/pubmed/9182680
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author Tough, David F.
Sun, Siquan
Sprent, Jonathan
author_facet Tough, David F.
Sun, Siquan
Sprent, Jonathan
author_sort Tough, David F.
collection PubMed
description Lipopolysaccharide (LPS) from gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other APC. We show here that, under in vivo conditions, LPS also induces strong stimulation of T cells. As manifested by CD69 upregulation, LPS injection stimulates both CD4 and CD8(+) T cells, and, at high doses, stimulates naive (CD44(lo)) cells as well as memory (CD44(hi)) cells. However, in terms of cell division, the response of T cells after LPS injection is limited to the CD44(hi) subset of CD8(+) cells. In contrast with B cells, proliferative responses of CD44(hi) CD8(+) cells require only very low doses of LPS (10 ng). Based on studies with LPS-nonresponder and gene-knockout mice, LPS-induced proliferation of CD44(hi) CD8(+) cells appears to operate via an indirect pathway involving LPS stimulation of APC and release of type I (α, β) interferon (IFN-I). Similar selective stimulation of CD44(hi) CD8(+) cells occurs in viral infections and after injection of IFN-I, implying a common mechanism. Hence, intermittent exposure to pathogens (gram-negative bacteria and viruses) could contribute to the high background proliferation of memory–phenotype CD8(+) cells found in normal animals.
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spelling pubmed-21963472008-04-16 T Cell Stimulation In Vivo by Lipopolysaccharide (LPS) Tough, David F. Sun, Siquan Sprent, Jonathan J Exp Med Article Lipopolysaccharide (LPS) from gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other APC. We show here that, under in vivo conditions, LPS also induces strong stimulation of T cells. As manifested by CD69 upregulation, LPS injection stimulates both CD4 and CD8(+) T cells, and, at high doses, stimulates naive (CD44(lo)) cells as well as memory (CD44(hi)) cells. However, in terms of cell division, the response of T cells after LPS injection is limited to the CD44(hi) subset of CD8(+) cells. In contrast with B cells, proliferative responses of CD44(hi) CD8(+) cells require only very low doses of LPS (10 ng). Based on studies with LPS-nonresponder and gene-knockout mice, LPS-induced proliferation of CD44(hi) CD8(+) cells appears to operate via an indirect pathway involving LPS stimulation of APC and release of type I (α, β) interferon (IFN-I). Similar selective stimulation of CD44(hi) CD8(+) cells occurs in viral infections and after injection of IFN-I, implying a common mechanism. Hence, intermittent exposure to pathogens (gram-negative bacteria and viruses) could contribute to the high background proliferation of memory–phenotype CD8(+) cells found in normal animals. The Rockefeller University Press 1997-06-16 /pmc/articles/PMC2196347/ /pubmed/9182680 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tough, David F.
Sun, Siquan
Sprent, Jonathan
T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)
title T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)
title_full T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)
title_fullStr T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)
title_full_unstemmed T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)
title_short T Cell Stimulation In Vivo by Lipopolysaccharide (LPS)
title_sort t cell stimulation in vivo by lipopolysaccharide (lps)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196347/
https://www.ncbi.nlm.nih.gov/pubmed/9182680
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