Cargando…

Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor

The earliest lymphoid precursor population in the adult mouse thymus had previously been shown to produce not only T cells, but also dendritic cell (DC) progeny on transfer to irradiated recipients. In this study, culture of these isolated thymic precursors with a mixture of cytokines induced them t...

Descripción completa

Detalles Bibliográficos
Autores principales: Saunders, Dolores, Lucas, Karen, Ismaili, Jamila, Wu, Li, Maraskovsky, Eugene, Dunn, Ashley, Shortman, Ken
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196371/
https://www.ncbi.nlm.nih.gov/pubmed/8976174
_version_ 1782148051375226880
author Saunders, Dolores
Lucas, Karen
Ismaili, Jamila
Wu, Li
Maraskovsky, Eugene
Dunn, Ashley
Shortman, Ken
author_facet Saunders, Dolores
Lucas, Karen
Ismaili, Jamila
Wu, Li
Maraskovsky, Eugene
Dunn, Ashley
Shortman, Ken
author_sort Saunders, Dolores
collection PubMed
description The earliest lymphoid precursor population in the adult mouse thymus had previously been shown to produce not only T cells, but also dendritic cell (DC) progeny on transfer to irradiated recipients. In this study, culture of these isolated thymic precursors with a mixture of cytokines induced them to proliferate and to differentiate to DC, but not to T lineage cells. At least 70% of the individual precursors had the capacity to form DC. The resultant DC were as effective as normal thymic DC in the functional test of T cell stimulation in mixed leukocyte cultures. The cultured DC also expressed high levels of class I and class II major histocompatibility complex, together with CD11c, DEC-205, CD80, and CD86, markers characteristic of mature DC in general. However, they did not express CD8α or BP-1, markers characteristic of normal thymic DC. The optimized mixture of five to seven cytokines required for DC development from these thymic precursors did not include granulocyte/macrophage colony stimulating factor (GM-CSF), usually required for DC development in culture. The addition of anti–GM-CSF antibody or the use of precursors from GM-CSF–deficient mice did not prevent DC development. Addition of GM-CSF was without effect on DC yield when interleukin (IL) 3 and IL-7 were present, although some stimulation by GM-CSF was noted in their absence. In contrast, DC development was enhanced by addition of the Flt3/Flk2 ligand, in line with the effects of the administration of this cytokine in vivo. The results indicate that the development of a particular lineage of DC, probably those of lymphoid precursor origin, may be independent of the myeloid hormone GM-CSF.
format Text
id pubmed-2196371
institution National Center for Biotechnology Information
language English
publishDate 1996
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21963712008-04-16 Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor Saunders, Dolores Lucas, Karen Ismaili, Jamila Wu, Li Maraskovsky, Eugene Dunn, Ashley Shortman, Ken J Exp Med Article The earliest lymphoid precursor population in the adult mouse thymus had previously been shown to produce not only T cells, but also dendritic cell (DC) progeny on transfer to irradiated recipients. In this study, culture of these isolated thymic precursors with a mixture of cytokines induced them to proliferate and to differentiate to DC, but not to T lineage cells. At least 70% of the individual precursors had the capacity to form DC. The resultant DC were as effective as normal thymic DC in the functional test of T cell stimulation in mixed leukocyte cultures. The cultured DC also expressed high levels of class I and class II major histocompatibility complex, together with CD11c, DEC-205, CD80, and CD86, markers characteristic of mature DC in general. However, they did not express CD8α or BP-1, markers characteristic of normal thymic DC. The optimized mixture of five to seven cytokines required for DC development from these thymic precursors did not include granulocyte/macrophage colony stimulating factor (GM-CSF), usually required for DC development in culture. The addition of anti–GM-CSF antibody or the use of precursors from GM-CSF–deficient mice did not prevent DC development. Addition of GM-CSF was without effect on DC yield when interleukin (IL) 3 and IL-7 were present, although some stimulation by GM-CSF was noted in their absence. In contrast, DC development was enhanced by addition of the Flt3/Flk2 ligand, in line with the effects of the administration of this cytokine in vivo. The results indicate that the development of a particular lineage of DC, probably those of lymphoid precursor origin, may be independent of the myeloid hormone GM-CSF. The Rockefeller University Press 1996-12-01 /pmc/articles/PMC2196371/ /pubmed/8976174 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Saunders, Dolores
Lucas, Karen
Ismaili, Jamila
Wu, Li
Maraskovsky, Eugene
Dunn, Ashley
Shortman, Ken
Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor
title Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor
title_full Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor
title_fullStr Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor
title_full_unstemmed Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor
title_short Dendritic Cell Development in Culture from Thymic Precursor Cells in the Absence of Granulocyte/Macrophage Colony-stimulating Factor
title_sort dendritic cell development in culture from thymic precursor cells in the absence of granulocyte/macrophage colony-stimulating factor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196371/
https://www.ncbi.nlm.nih.gov/pubmed/8976174
work_keys_str_mv AT saundersdolores dendriticcelldevelopmentinculturefromthymicprecursorcellsintheabsenceofgranulocytemacrophagecolonystimulatingfactor
AT lucaskaren dendriticcelldevelopmentinculturefromthymicprecursorcellsintheabsenceofgranulocytemacrophagecolonystimulatingfactor
AT ismailijamila dendriticcelldevelopmentinculturefromthymicprecursorcellsintheabsenceofgranulocytemacrophagecolonystimulatingfactor
AT wuli dendriticcelldevelopmentinculturefromthymicprecursorcellsintheabsenceofgranulocytemacrophagecolonystimulatingfactor
AT maraskovskyeugene dendriticcelldevelopmentinculturefromthymicprecursorcellsintheabsenceofgranulocytemacrophagecolonystimulatingfactor
AT dunnashley dendriticcelldevelopmentinculturefromthymicprecursorcellsintheabsenceofgranulocytemacrophagecolonystimulatingfactor
AT shortmanken dendriticcelldevelopmentinculturefromthymicprecursorcellsintheabsenceofgranulocytemacrophagecolonystimulatingfactor