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RNA-mediated interaction of Cajal bodies and U2 snRNA genes

Cajal bodies (CBs) are nuclear structures involved in RNA metabolism that accumulate high concentrations of small nuclear ribonucleoproteins (snRNPs). Notably, CBs preferentially associate with specific genomic loci in interphase human cells, including several snRNA and histone gene clusters. To unc...

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Detalles Bibliográficos
Autores principales: Frey, Mark R., Matera, A. Gregory
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196410/
https://www.ncbi.nlm.nih.gov/pubmed/11489914
http://dx.doi.org/10.1083/jcb.200105084
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author Frey, Mark R.
Matera, A. Gregory
author_facet Frey, Mark R.
Matera, A. Gregory
author_sort Frey, Mark R.
collection PubMed
description Cajal bodies (CBs) are nuclear structures involved in RNA metabolism that accumulate high concentrations of small nuclear ribonucleoproteins (snRNPs). Notably, CBs preferentially associate with specific genomic loci in interphase human cells, including several snRNA and histone gene clusters. To uncover functional elements involved in the interaction of genes and CBs, we analyzed the expression and subcellular localization of stably transfected artificial arrays of U2 snRNA genes. Although promoter substitution arrays colocalized with CBs, constructs containing intragenic deletions did not. Additional experiments identified factors within CBs that are important for association with the native U2 genes. Inhibition of nuclear export or targeted degradation of U2 snRNPs caused a marked decrease in the levels of U2 snRNA in CBs and strongly disrupted the interaction with U2 genes. Together, the results illustrate a specific requirement for both the snRNA transcripts as well as the presence of snRNPs (or snRNP proteins) within CBs. Our data thus provide significant insight into the mechanism of CB interaction with snRNA loci, strengthening the putative role for this nuclear suborganelle in snRNP biogenesis.
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spelling pubmed-21964102008-05-01 RNA-mediated interaction of Cajal bodies and U2 snRNA genes Frey, Mark R. Matera, A. Gregory J Cell Biol Article Cajal bodies (CBs) are nuclear structures involved in RNA metabolism that accumulate high concentrations of small nuclear ribonucleoproteins (snRNPs). Notably, CBs preferentially associate with specific genomic loci in interphase human cells, including several snRNA and histone gene clusters. To uncover functional elements involved in the interaction of genes and CBs, we analyzed the expression and subcellular localization of stably transfected artificial arrays of U2 snRNA genes. Although promoter substitution arrays colocalized with CBs, constructs containing intragenic deletions did not. Additional experiments identified factors within CBs that are important for association with the native U2 genes. Inhibition of nuclear export or targeted degradation of U2 snRNPs caused a marked decrease in the levels of U2 snRNA in CBs and strongly disrupted the interaction with U2 genes. Together, the results illustrate a specific requirement for both the snRNA transcripts as well as the presence of snRNPs (or snRNP proteins) within CBs. Our data thus provide significant insight into the mechanism of CB interaction with snRNA loci, strengthening the putative role for this nuclear suborganelle in snRNP biogenesis. The Rockefeller University Press 2001-08-06 /pmc/articles/PMC2196410/ /pubmed/11489914 http://dx.doi.org/10.1083/jcb.200105084 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Frey, Mark R.
Matera, A. Gregory
RNA-mediated interaction of Cajal bodies and U2 snRNA genes
title RNA-mediated interaction of Cajal bodies and U2 snRNA genes
title_full RNA-mediated interaction of Cajal bodies and U2 snRNA genes
title_fullStr RNA-mediated interaction of Cajal bodies and U2 snRNA genes
title_full_unstemmed RNA-mediated interaction of Cajal bodies and U2 snRNA genes
title_short RNA-mediated interaction of Cajal bodies and U2 snRNA genes
title_sort rna-mediated interaction of cajal bodies and u2 snrna genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196410/
https://www.ncbi.nlm.nih.gov/pubmed/11489914
http://dx.doi.org/10.1083/jcb.200105084
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