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Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole
The localization of Oskar at the posterior pole of the Drosophila oocyte induces the assembly of the pole plasm and therefore defines where the abdomen and germ cells form in the embryo. This localization is achieved by the targeting of oskar mRNA to the posterior and the localized activation of its...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196428/ https://www.ncbi.nlm.nih.gov/pubmed/11481346 http://dx.doi.org/10.1083/jcb.200105056 |
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author | van Eeden, Fredericus J.M. Palacios, Isabel M. Petronczki, Mark Weston, Matthew J.D. St Johnston, Daniel |
author_facet | van Eeden, Fredericus J.M. Palacios, Isabel M. Petronczki, Mark Weston, Matthew J.D. St Johnston, Daniel |
author_sort | van Eeden, Fredericus J.M. |
collection | PubMed |
description | The localization of Oskar at the posterior pole of the Drosophila oocyte induces the assembly of the pole plasm and therefore defines where the abdomen and germ cells form in the embryo. This localization is achieved by the targeting of oskar mRNA to the posterior and the localized activation of its translation. oskar mRNA seems likely to be actively transported along microtubules, since its localization requires both an intact microtubule cytoskeleton and the plus end–directed motor kinesin I, but nothing is known about how the RNA is coupled to the motor. Here, we describe barentsz, a novel gene required for the localization of oskar mRNA. In contrast to all other mutations that disrupt this process, barentsz-null mutants completely block the posterior localization of oskar mRNA without affecting bicoid and gurken mRNA localization, the organization of the microtubules, or subsequent steps in pole plasm assembly. Surprisingly, most mutant embryos still form an abdomen, indicating that oskar mRNA localization is partially redundant with the translational control. Barentsz protein colocalizes to the posterior with oskar mRNA, and this localization is oskar mRNA dependent. Thus, Barentsz is essential for the posterior localization of oskar mRNA and behaves as a specific component of the oskar RNA transport complex. |
format | Text |
id | pubmed-2196428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21964282008-05-01 Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole van Eeden, Fredericus J.M. Palacios, Isabel M. Petronczki, Mark Weston, Matthew J.D. St Johnston, Daniel J Cell Biol Article The localization of Oskar at the posterior pole of the Drosophila oocyte induces the assembly of the pole plasm and therefore defines where the abdomen and germ cells form in the embryo. This localization is achieved by the targeting of oskar mRNA to the posterior and the localized activation of its translation. oskar mRNA seems likely to be actively transported along microtubules, since its localization requires both an intact microtubule cytoskeleton and the plus end–directed motor kinesin I, but nothing is known about how the RNA is coupled to the motor. Here, we describe barentsz, a novel gene required for the localization of oskar mRNA. In contrast to all other mutations that disrupt this process, barentsz-null mutants completely block the posterior localization of oskar mRNA without affecting bicoid and gurken mRNA localization, the organization of the microtubules, or subsequent steps in pole plasm assembly. Surprisingly, most mutant embryos still form an abdomen, indicating that oskar mRNA localization is partially redundant with the translational control. Barentsz protein colocalizes to the posterior with oskar mRNA, and this localization is oskar mRNA dependent. Thus, Barentsz is essential for the posterior localization of oskar mRNA and behaves as a specific component of the oskar RNA transport complex. The Rockefeller University Press 2001-08-06 /pmc/articles/PMC2196428/ /pubmed/11481346 http://dx.doi.org/10.1083/jcb.200105056 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article van Eeden, Fredericus J.M. Palacios, Isabel M. Petronczki, Mark Weston, Matthew J.D. St Johnston, Daniel Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole |
title | Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole |
title_full | Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole |
title_fullStr | Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole |
title_full_unstemmed | Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole |
title_short | Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole |
title_sort | barentsz is essential for the posterior localization of oskar mrna and colocalizes with it to the posterior pole |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196428/ https://www.ncbi.nlm.nih.gov/pubmed/11481346 http://dx.doi.org/10.1083/jcb.200105056 |
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