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Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions

Connexin 43 (Cx43α1) gap junction has been shown to have an essential role in mediating functional coupling of neural crest cells and in modulating neural crest cell migration. Here, we showed that N-cadherin and wnt1 are required for efficient dye coupling but not for the expression of Cx43α1 gap j...

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Autores principales: Xu, X., Li, W.E.I., Huang, G.Y., Meyer, R., Chen, T., Luo, Y., Thomas, M.P., Radice, G.L., Lo, C.W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196865/
https://www.ncbi.nlm.nih.gov/pubmed/11449002
http://dx.doi.org/10.1083/jcb.200105047
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author Xu, X.
Li, W.E.I.
Huang, G.Y.
Meyer, R.
Chen, T.
Luo, Y.
Thomas, M.P.
Radice, G.L.
Lo, C.W.
author_facet Xu, X.
Li, W.E.I.
Huang, G.Y.
Meyer, R.
Chen, T.
Luo, Y.
Thomas, M.P.
Radice, G.L.
Lo, C.W.
author_sort Xu, X.
collection PubMed
description Connexin 43 (Cx43α1) gap junction has been shown to have an essential role in mediating functional coupling of neural crest cells and in modulating neural crest cell migration. Here, we showed that N-cadherin and wnt1 are required for efficient dye coupling but not for the expression of Cx43α1 gap junctions in neural crest cells. Cell motility was found to be altered in the N-cadherin–deficient neural crest cells, but the alterations were different from that elicited by Cx43α1 deficiency. In contrast, wnt1-deficient neural crest cells showed no discernible change in cell motility. These observations suggest that dye coupling may not be a good measure of gap junction communication relevant to motility. Alternatively, Cx43α1 may serve a novel function in motility. We observed that p120 catenin (p120ctn), an Armadillo protein known to modulate cell motility, is colocalized not only with N-cadherin but also with Cx43α1. Moreover, the subcellular distribution of p120ctn was altered with N-cadherin or Cx43α1 deficiency. Based on these findings, we propose a model in which Cx43α1 and N-cadherin may modulate neural crest cell motility by engaging in a dynamic cross-talk with the cell's locomotory apparatus through p120ctn signaling.
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spelling pubmed-21968652008-05-01 Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions Xu, X. Li, W.E.I. Huang, G.Y. Meyer, R. Chen, T. Luo, Y. Thomas, M.P. Radice, G.L. Lo, C.W. J Cell Biol Research Articles Connexin 43 (Cx43α1) gap junction has been shown to have an essential role in mediating functional coupling of neural crest cells and in modulating neural crest cell migration. Here, we showed that N-cadherin and wnt1 are required for efficient dye coupling but not for the expression of Cx43α1 gap junctions in neural crest cells. Cell motility was found to be altered in the N-cadherin–deficient neural crest cells, but the alterations were different from that elicited by Cx43α1 deficiency. In contrast, wnt1-deficient neural crest cells showed no discernible change in cell motility. These observations suggest that dye coupling may not be a good measure of gap junction communication relevant to motility. Alternatively, Cx43α1 may serve a novel function in motility. We observed that p120 catenin (p120ctn), an Armadillo protein known to modulate cell motility, is colocalized not only with N-cadherin but also with Cx43α1. Moreover, the subcellular distribution of p120ctn was altered with N-cadherin or Cx43α1 deficiency. Based on these findings, we propose a model in which Cx43α1 and N-cadherin may modulate neural crest cell motility by engaging in a dynamic cross-talk with the cell's locomotory apparatus through p120ctn signaling. The Rockefeller University Press 2001-07-09 /pmc/articles/PMC2196865/ /pubmed/11449002 http://dx.doi.org/10.1083/jcb.200105047 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Xu, X.
Li, W.E.I.
Huang, G.Y.
Meyer, R.
Chen, T.
Luo, Y.
Thomas, M.P.
Radice, G.L.
Lo, C.W.
Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions
title Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions
title_full Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions
title_fullStr Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions
title_full_unstemmed Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions
title_short Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions
title_sort modulation of mouse neural crest cell motility by n-cadherin and connexin 43 gap junctions
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196865/
https://www.ncbi.nlm.nih.gov/pubmed/11449002
http://dx.doi.org/10.1083/jcb.200105047
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