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Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins
XendoU is the first endoribonuclease described in higher eukaryotes as being involved in the endonucleolytic processing of intron-encoded small nucleolar RNAs. It is conserved among eukaryotes and its viral homologue is essential in SARS replication and transcription. The large-scale purification an...
Autores principales: | Renzi, Fabiana, Panetta, Gianna, Vallone, Beatrice, Brunori, Maurizio, Arceci, Massimo, Bozzoni, Irene, Laneve, Pietro, Caffarelli, Elisa |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2197201/ https://www.ncbi.nlm.nih.gov/pubmed/16511328 http://dx.doi.org/10.1107/S1744309106006373 |
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