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Maintenance of Golgi structure and function depends on the integrity of ER export

The Golgi apparatus comprises an enormous array of components that generate its unique architecture and function within cells. Here, we use quantitative fluorescence imaging techniques and ultrastructural analysis to address whether the Golgi apparatus is a steady-state or a stable organelle. We fou...

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Autores principales: Ward, Theresa H., Polishchuk, Roman S., Caplan, Steve, Hirschberg, Koret, Lippincott-Schwartz, Jennifer
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2198855/
https://www.ncbi.nlm.nih.gov/pubmed/11706049
http://dx.doi.org/10.1083/jcb.200107045
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author Ward, Theresa H.
Polishchuk, Roman S.
Caplan, Steve
Hirschberg, Koret
Lippincott-Schwartz, Jennifer
author_facet Ward, Theresa H.
Polishchuk, Roman S.
Caplan, Steve
Hirschberg, Koret
Lippincott-Schwartz, Jennifer
author_sort Ward, Theresa H.
collection PubMed
description The Golgi apparatus comprises an enormous array of components that generate its unique architecture and function within cells. Here, we use quantitative fluorescence imaging techniques and ultrastructural analysis to address whether the Golgi apparatus is a steady-state or a stable organelle. We found that all classes of Golgi components are dynamically associated with this organelle, contrary to the prediction of the stable organelle model. Enzymes and recycling components are continuously exiting and reentering the Golgi apparatus by membrane trafficking pathways to and from the ER, whereas Golgi matrix proteins and coatomer undergo constant, rapid exchange between membrane and cytoplasm. When ER to Golgi transport is inhibited without disrupting COPII-dependent ER export machinery (by brefeldin A treatment or expression of Arf1[T31N]), the Golgi structure disassembles, leaving no residual Golgi membranes. Rather, all Golgi components redistribute into the ER, the cytoplasm, or to ER exit sites still active for recruitment of selective membrane-bound and peripherally associated cargos. A similar phenomenon is induced by the constitutively active Sar1[H79G] mutant, which has the additional effect of causing COPII-associated membranes to cluster to a juxtanuclear region. In cells expressing Sar1[T39N], a constitutively inactive form of Sar1 that completely disrupts ER exit sites, Golgi glycosylation enzymes, matrix, and itinerant proteins all redistribute to the ER. These results argue against the hypothesis that the Golgi apparatus contains stable components that can serve as a template for its biogenesis. Instead, they suggest that the Golgi complex is a dynamic, steady-state system, whose membranes can be nucleated and are maintained by the activities of the Sar1–COPII and Arf1–coatomer systems.
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spelling pubmed-21988552008-05-01 Maintenance of Golgi structure and function depends on the integrity of ER export Ward, Theresa H. Polishchuk, Roman S. Caplan, Steve Hirschberg, Koret Lippincott-Schwartz, Jennifer J Cell Biol Article The Golgi apparatus comprises an enormous array of components that generate its unique architecture and function within cells. Here, we use quantitative fluorescence imaging techniques and ultrastructural analysis to address whether the Golgi apparatus is a steady-state or a stable organelle. We found that all classes of Golgi components are dynamically associated with this organelle, contrary to the prediction of the stable organelle model. Enzymes and recycling components are continuously exiting and reentering the Golgi apparatus by membrane trafficking pathways to and from the ER, whereas Golgi matrix proteins and coatomer undergo constant, rapid exchange between membrane and cytoplasm. When ER to Golgi transport is inhibited without disrupting COPII-dependent ER export machinery (by brefeldin A treatment or expression of Arf1[T31N]), the Golgi structure disassembles, leaving no residual Golgi membranes. Rather, all Golgi components redistribute into the ER, the cytoplasm, or to ER exit sites still active for recruitment of selective membrane-bound and peripherally associated cargos. A similar phenomenon is induced by the constitutively active Sar1[H79G] mutant, which has the additional effect of causing COPII-associated membranes to cluster to a juxtanuclear region. In cells expressing Sar1[T39N], a constitutively inactive form of Sar1 that completely disrupts ER exit sites, Golgi glycosylation enzymes, matrix, and itinerant proteins all redistribute to the ER. These results argue against the hypothesis that the Golgi apparatus contains stable components that can serve as a template for its biogenesis. Instead, they suggest that the Golgi complex is a dynamic, steady-state system, whose membranes can be nucleated and are maintained by the activities of the Sar1–COPII and Arf1–coatomer systems. The Rockefeller University Press 2001-11-12 /pmc/articles/PMC2198855/ /pubmed/11706049 http://dx.doi.org/10.1083/jcb.200107045 Text en Copyright © 2001, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ward, Theresa H.
Polishchuk, Roman S.
Caplan, Steve
Hirschberg, Koret
Lippincott-Schwartz, Jennifer
Maintenance of Golgi structure and function depends on the integrity of ER export
title Maintenance of Golgi structure and function depends on the integrity of ER export
title_full Maintenance of Golgi structure and function depends on the integrity of ER export
title_fullStr Maintenance of Golgi structure and function depends on the integrity of ER export
title_full_unstemmed Maintenance of Golgi structure and function depends on the integrity of ER export
title_short Maintenance of Golgi structure and function depends on the integrity of ER export
title_sort maintenance of golgi structure and function depends on the integrity of er export
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2198855/
https://www.ncbi.nlm.nih.gov/pubmed/11706049
http://dx.doi.org/10.1083/jcb.200107045
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